This work was supported by the National Natural Science Foundation of China (81170068) and the Scientific Research Foundation for Returned Scholars, Ministry of Education of China (2008-890).
Reduced DHPRα1S and RyR1 expression levels are associated with diaphragm contractile dysfunction during sepsis
Article first published online: 13 AUG 2013
Copyright © 2013 Wiley Periodicals, Inc.
Muscle & Nerve
Volume 48, Issue 5, pages 745–751, November 2013
How to Cite
Jiao, G.-Y., Hao, L.-Y., Gao, C.-E., Chen, L., Sun, X.-F., Yang, H.-L., Li, Y. and Dai, Y.-N. (2013), Reduced DHPRα1S and RyR1 expression levels are associated with diaphragm contractile dysfunction during sepsis. Muscle Nerve, 48: 745–751. doi: 10.1002/mus.23805
- Issue published online: 28 OCT 2013
- Article first published online: 13 AUG 2013
- Accepted manuscript online: 5 FEB 2013 08:18AM EST
- Manuscript Accepted: 24 JAN 2013
- diaphragm muscle;
- excitation–contraction coupling;
Sepsis often causes diaphragm contractile dysfunction. Dihydropyridine receptors (DHPRα1s and DHPRα1c) and ryanodine receptors (RyR1, RyR2, and RyR3) are essential for excitation–contraction coupling in striated muscles. However, their expression in diaphragm during sepsis have not been explored. Methods: Eight rats received endotoxin, and 8 more rats received placebo. After 24 hours, 3) diaphragm isometric contractile force was measured. The mRNA and protein levels of DHPRs and RyRs in diaphragm muscles were determined.Results: Sepsis weakened diaphragm contractile function. The expression levels of DHPRα1s and RyR1 were significantly lower in septic rats than in control rats. The expression levels of DHPRα1c and RyR3 were unaffected by sepsis. RyR2 was undetectable at both mRNA and protein levels in the control and sepsis groups. Conclusions: Weakened diaphragm contraction in the septic rats was associated with reduced mRNA and protein expression of DHPRα1s and RyR1, the isoforms of skeletal muscles. Muscle Nerve 48:745–751, 2013