This study was sponsored by Cytokinetics, Inc. F.I.M. and J.R.J. are employees of Cytokinetics and own stock in Cytokinetics. K.R.W. has served as a consultant to Cytokinetics. Ms. Tibbs' present address is Department of Neural and Pain Sciences, Dental School, Program in Neuroscience, University of Maryland, Baltimore, MD 21201.
Fast skeletal muscle troponin activator in the dy2J muscular dystrophy model
Article first published online: 29 JUN 2013
© 2013 Wiley Periodicals, Inc.
Muscle & Nerve
Volume 48, Issue 2, pages 279–285, August 2013
How to Cite
Miciak, J. J., Warsing, L. C., Tibbs, M. E., JASPER, J. R., Jampel, S. B., Malik, F. I., Tankersley, C. and Wagner, K. R. (2013), Fast skeletal muscle troponin activator in the dy2J muscular dystrophy model. Muscle Nerve, 48: 279–285. doi: 10.1002/mus.23848
- Issue published online: 23 JUL 2013
- Article first published online: 29 JUN 2013
- Accepted manuscript online: 20 MAR 2013 04:22AM EST
- Manuscript Accepted: 12 MAR 2013
- muscular dystrophy;
- troponin complex
Introduction: Tirasemtiv is a novel small molecule activator of the fast skeletal muscle troponin complex that produces sensitization of the sarcomere to calcium. Tirasemtiv is currently in Phase II clinical trials for neuromuscular disease. Methods: We conducted a blinded, randomized, placebo-controlled preclinical study of the effect of tirasemtiv on forearm grip strength, endurance, respiratory physiology, and muscle pathology in adequate sample sizes of the Lama2dy-2J mouse model of congenital muscular dystrophy. Results: Mice receiving a high dose of tirasemtiv had significantly higher muscle fiber cross-sectional area and respiratory response to CO2 stimulation at 16 weeks than mice on low dose or placebo. There were no changes in muscle pathology, serum creatine kinase, strength, endurance, or respiration following long-term treatment. Conclusions: We conclude that tirasemtiv influences the structure of the skeletal muscle fiber in this model of muscular dystrophy but does not impact muscle function, as evaluated in this study. Muscle Nerve, 48: 279–285, 2013