This study was supported by the National Institutes of Health (R01 AG020532, R15 AG032127, and R01 AG038576).
Local anesthetic effects on gene transcription in human skeletal muscle biopsies
Article first published online: 30 AUG 2013
Copyright © 2013 Wiley Periodicals, Inc.
Muscle & Nerve
Volume 48, Issue 4, pages 591–593, October 2013
How to Cite
Trappe, T. A., Standley, R. A., Liu, S. Z., Jemiolo, B., Trappe, S. W. and Harber, M. P. (2013), Local anesthetic effects on gene transcription in human skeletal muscle biopsies. Muscle Nerve, 48: 591–593. doi: 10.1002/mus.23860
- Issue published online: 23 SEP 2013
- Article first published online: 30 AUG 2013
- Accepted manuscript online: 2 APR 2013 04:56AM EST
- Manuscript Accepted: 26 MAR 2013
Introduction: We examined if epinephrine in the local anesthetic to help control incision-related bleeding interferes with molecular measurements obtained with the Duchenne-Bergström percutaneous needle biopsy technique for sampling human skeletal muscle. Methods: Three groups received 2.5–3.0 ml of 1% lidocaine in 2 injections: (1) 0.5–1.0 ml superficially, which varied among the groups according to (i) −Epi; intra- and subcutaneous without epinephrine, (ii) +Epi −Fascia; intra- and subcutaneous with epinephrine, avoiding the fascia, and (iii) +Epi +Fascia; intra- and subcutaneous with epinephrine, directing a small amount (∽0.2 ml) into the fascia area; and (2) ∽2.0 ml without epinephrine into the fascia area for all subjects. A muscle biopsy was obtained 5–10 min later for IL-6 and MuRF-1 mRNA levels. Results: IL-6 mRNA levels were low in −Epi and +Epi −Fascia, but ∽300-fold higher in +Epi +Fascia. MuRF-1 mRNA levels were similar among the groups. Conclusions: Lidocaine with epinephrine can confound intramuscular measurements from needle biopsies, but this can be avoided with a careful injection approach. Muscle Nerve 48: 591–593, 2013