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Understanding the beneficial effects of doxycycline on the dystrophic phenotype of the mdx mouse

Authors

  • Juliano Alves Pereira MsSci,

    1. Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), São Paulo, Brazil
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  • Cintia Yuri Matsumura DSci,

    1. Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), São Paulo, Brazil
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  • Elaine Minatel PhD,

    1. Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), São Paulo, Brazil
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  • Maria Julia Marques PhD,

    1. Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), São Paulo, Brazil
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  • Humberto Santo Neto PhD

    Corresponding author
    1. Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), São Paulo, Brazil
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  • This work was supported by grant from the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP; 04/15526-9, 08/58491-1, and 11/51697-6). H.S.N. and M.J.M. were recipients of fellowships from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; 301306/2010-9, 302006/2009-5, and 474708/06-3). J.A.P. and C.Y.M. were recipients of CNPq and FAPESP fellowships (142935/2011-5 and 08/54775-5).

ABSTRACT

Introduction: The purpose of this study was to better understand the beneficial effects of doxycycline on the dystrophic muscles of the mdx mouse. Methods: Doxycycline (DOX) was administered for 36 days, starting on postnatal day 0, via drinking water. Untreated mdx mice received plain water for the same period and served as a control group. Results: DOX decreased the levels of metalloproteinase-9 and tumor necrosis factor-alpha in the biceps brachii and diaphragm of the mdx mice. It also reduced the total amount of calcium in the muscles studied, concomitant with an increase in the levels of calsequestrin 1. Conclusions: The results show that DOX can affect factors that are important in dystrophic pathogenesis and highlight its potential as a readily accessible therapy in clinical trials for treatment of Duchenne muscular dystrophy. Muscle Nerve 50:283–286, 2014

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