The first 2 authors (A.B.R. and J.C.L.) contributed equally to this study.
A novel protocol for assessing exercise performance and dystropathophysiology in the mdx mouse
Article first published online: 5 AUG 2014
© 2014 Wiley Periodicals, Inc.
Muscle & Nerve
Volume 50, Issue 4, pages 541–548, October 2014
How to Cite
Rocco, A. B., Levalley, J. C., Eldridge, J. A., Marsh, S. A. and Rodgers, B. D. (2014), A novel protocol for assessing exercise performance and dystropathophysiology in the mdx mouse. Muscle Nerve, 50: 541–548. doi: 10.1002/mus.24184
These studies were supported by a grant from the Muscular Dystrophy Association (216602 to B.D.R., S.A.M., and the Washington Center for Muscle Biology).
- Issue published online: 22 SEP 2014
- Article first published online: 5 AUG 2014
- Accepted manuscript online: 22 JAN 2014 06:05AM EST
- Manuscript Accepted: 15 JAN 2014
- Manuscript Revised: 6 JAN 2014
- Manuscript Received: 5 SEP 2013
- Duchenne muscular dystrophy;
- respiratory performance;
Introduction: Dystrophinopathy in the young mdx mouse model of Duchenne muscular dystrophy is comparatively mild, requires induction, and is rarely assessed with tests of systemic muscle function in whole animals. Methods: A modified TREAT-NMD induction protocol was used to evaluate respiratory and exercise performance, starting and ending with maximum oxygen consumption (VO2max) tests. Results: The initial and/or final VO2max, time to exhaustion, speed at exhaustion, and total expended calories were significantly lower in mdx mice. Episodic VO2 and VCO2 fluctuations occurred during training and resulted in dissociated patterns of VO2 and respiratory exchange ratio (RER). These fluctuations further resulted in significantly greater VO2 coefficient of variation and RER values and lower minimal VO2 values. Conclusions: Quantifying respiratory performance during exercise is a potentially useful means for studying pathophysiology in mdx mice, as it assesses intact animals over time, is more sensitive than some histological markers, and assesses systemic muscle function. Muscle Nerve 50: 541–548, 2014