Additional Supporting Information may be found in the online version of this article.

mus24220-sup-0001-suppinfo.doc59KSupporting Information
mus24220-sup-0002-suppfig1.tif2602KSupporting Information Figure 1. Optimization of tissue homogenization for Immunoblotting dysferlin. (A) Homogenates of the right and left TA muscles of 2 A/WySnJ mice were prepared under several different conditions to identify conditions that give the most complete extraction of dysferlin with the least background noise. (B) The use of RIPA buffer and sonication result in the most complete extraction of dysferlin with the least amount of background noise.
mus24220-sup-0003-suppfig2.tif3563KSupporting Information Figure 2. Immunogold labeling of dysferlin is specific and places dysferlin at the ER, SR, Golgi networks. (A,B) Immunogold labeling performed with the Hamlet antibody gives clear and defined labeling of dysferlin and suggests that dysferlin is enriched at membranes close to the Z-disk, which have been reported to be part of the ER, SR, and Golgi networks. Labeling with non specific mouse IgG does not show any gold particles confirming that dysferlin labeling with Hamlet is specific. (C) Results from all pairwise comparisons for the statistical analyses shown in Figure 1I are provided.
mus24220-sup-0004-suppfig3.tif7701KSupporting Information Figure 3. Dysferlin is enriched in membranes close to the Z-disk. (A,B) The highest percentage of gold particles bound to the Hamlet antibody are present at the outer and inner Z-disk.

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