Heterogeneity of type 1 skeletal muscle fibers revealed by monoclonal antibody to slow myosin
Article first published online: 13 OCT 2004
Copyright © 1984 John Wiley & Sons, Inc.
Muscle & Nerve
Volume 7, Issue 5, pages 380–387, June 1984
How to Cite
Shafiq, S. A., Shimizu, T. and Fischman, D. A. (1984), Heterogeneity of type 1 skeletal muscle fibers revealed by monoclonal antibody to slow myosin. Muscle Nerve, 7: 380–387. doi: 10.1002/mus.880070507
- Issue published online: 13 OCT 2004
- Article first published online: 13 OCT 2004
- Manuscript Accepted: 27 SEP 1983
- Manuscript Received: 20 JUL 1983
Monoclonal antibodies (McAbs) were generated against slow myosin from the chicken anterior latissimus dorsi (ALD) muscle and their reactivity was checked against fast (pectoralis), slow (ALD), cardiac (ventricular), and smooth (gizzard) myosins by radioimmunoassay (RIA), immunoautoradiography (immunoblots), and indirect immunofluorescence techniques. In RIAs, the McAb (ALD-58) described in this article reacted specifically with slow myosin, with only a weak cross-reactivity to cardiac myosin. In immunoblots against whole muscle homogenates and purified myosins, it bound selectively to the 200 Kd myosin heavy chain band. The ALD-58 antibody stained the fibers of the ALD muscle uniformly but gave three grades of reactions (strong, weak, and negative), with histochemically identified type 1 fibers of sartorius and gastrocnemius muscles demonstrating the immunological heterogeneity of myosins in type 1 skeletal muscle fibers.