[3H]Nitrendipine receptors as markers of a class of putative voltage-sensitive Ca2+ channels in normal human skeletal muscle and in muscle from duchenne muscular dystrophy patients
Article first published online: 13 OCT 2004
Copyright © 1986 John Wiley & Sons, Inc.
Muscle & Nerve
Volume 9, Issue 2, pages 148–151, February 1986
How to Cite
Desnuelle, C., Renaud, J.-F., Delpont, E., Serratrice, G., Lazdunski, R. D. and Lazdunski, M. (1986), [3H]Nitrendipine receptors as markers of a class of putative voltage-sensitive Ca2+ channels in normal human skeletal muscle and in muscle from duchenne muscular dystrophy patients. Muscle Nerve, 9: 148–151. doi: 10.1002/mus.880090207
- Issue published online: 13 OCT 2004
- Article first published online: 13 OCT 2004
- Manuscript Accepted: 24 JUN 1985
- Manuscript Received: 22 OCT 1984
Properties of nitrendipine receptors have been analyzed in skeletal muscle from normal young boys and boys with Duchenne muscular dystrophy (DMD). The dissociation constant (Kd) of the complex formed by nitrendipine with its specific receptors was 0.5 ± 0.1 nM in dystrophic muscle and 0.4 ± 0.1 nM in normal muscle. Maximum binding capacities Bmax were 403 ± 80 and 460 ± 60 fmol/mg protein in DMD and normal muscle, respectively. These results suggest that nitrendipine binding sites on nitrendipine-sensitive Ca2+ channel binding sites are not altered in Duchenne muscular dystrophy.