Expression of myoglobin gene in skeletal muscle of patients with neuromuscular diseases
Article first published online: 13 OCT 2004
Copyright © 1994 John Wiley & Sons, Inc.
Muscle & Nerve
Volume 17, Issue 7, pages 720–724, July 1994
How to Cite
Kawai, H., Naruo, T., Yoneda, K., Masuda, K., Mitsui, T. and Nishida, Y. (1994), Expression of myoglobin gene in skeletal muscle of patients with neuromuscular diseases. Muscle Nerve, 17: 720–724. doi: 10.1002/mus.880170703
- Issue published online: 13 OCT 2004
- Article first published online: 13 OCT 2004
- Manuscript Accepted: 2 JAN 1994
- myoglobin gene;
- myoglobin synthesis;
- Duchenne muscular dystrophy;
- amyotrophic lateral sclerosis
Expression of the myoglobin (Mb) gene in skeletal muscle was studied in patients with Duchenne muscular dystrophy (DMD), polymyositis (PM), or amyotrophic lateral sclerosis (ALS) by measuring Mb concentration by radioimmunoassay and Mb messenger ribonucleic acid (RNA) (MbmRNA) levels by Northern blot analysis. Mb concentrations in the muscle cells (Mb/noncollagenous protein) were decreased in patients with DMD, PM, or ALS. However, while Mb concentrations per MbmRNA content (Mb/MbmRNA) were decreased in DMD and PM patients, these values were normal in ALS patients. These results suggest that Mb synthesis is increased in muscles of DMD and PM patients, but is not sufficient to compensate for the excessive loss of Mb from the affected muscles, and that the synthesis is decreased in the muscles of ALS patients. © 1994 John Wiley & Sons, Inc.