Original Basic Science Article
Systemic oxybutynin decreases afferent activity of the pelvic nerve of the rat: New insights into the working mechanism of antimuscarinics
Article first published online: 21 DEC 2005
Copyright © 2005 Wiley-Liss, Inc.
Neurourology and Urodynamics
Volume 25, Issue 2, pages 156–161, March 2006
How to Cite
De Laet, K., De Wachter, S. and Wyndaele, J.-J. (2006), Systemic oxybutynin decreases afferent activity of the pelvic nerve of the rat: New insights into the working mechanism of antimuscarinics. Neurourol. Urodyn., 25: 156–161. doi: 10.1002/nau.20208
- Issue published online: 15 FEB 2006
- Article first published online: 21 DEC 2005
- Manuscript Accepted: 13 SEP 2005
- Manuscript Received: 10 JUL 2005
- University of Antwerp
- overactive bladder;
In a rat model, intravesical oxybutynin was recently shown to suppress pelvic afferent nerves. This study evaluates if a similar effect exists after systemic administration of oxybutynin.
Twenty-four single afferent bladder nerves were identified in 15 rats. Based on their conduction velocities they were grouped as C or Aδ fibers. Bladder filling parameters and afferent nerve spike rate were simultaneously recorded 30 min before administration of saline (nine fibers) or oxybutynin (15 fibers, 1 mg/kg), and again 30, 60, 90, 120, and 150 min after systemic saline or drug administration.
No change in C or Aδ afferent spike rate was observed after saline injection (P > 0.90). In the study group, a decrease in afferent activity was noted after systemic administration of oxybutynin for C fibers, which were statistically significant 90 (P < 0.004) and 120 min (P < 0.028) after drug delivery. After 150 min, the spike rate was still lower compared to the baseline filling, without reaching the level of significance (P > 0.09). For the Aδ fibers the decrease in afferent spike rate was already significant at 30 min (P < 0.005) and remained significant during all subsequent fillings (P < 0.012). To avoid a possible confounding influence of the bladder compliance, which increased significantly after injection of oxybutynin (P < 0.011), afferent activity during bladder filling was recalculated. Normalized afferent sensitivity of C and Aδ fibers decreased significantly after injection of oxybutynin. This means that the decrease in afferent spike rate is not the result of an increased compliance.
The findings of this study strongly suggest that oxybutynin directly or indirectly influences bladder sensory nerves, inhibiting the afferent part of the micturition reflex. Neurourol Urodynam. © 2005 Wiley-Liss, Inc.