No conflict of interest reported by the author(s).
Original Basic Science Article
Enhanced expression of mast cell growth factor and mast cell activation in the bladder following the resolution of trinitrobenzenesulfonic acid (TNBS) colitis in female rats†
Article first published online: 23 MAR 2007
Copyright © 2007 Wiley-Liss, Inc.
Neurourology and Urodynamics
Volume 26, Issue 6, pages 887–893, October 2007
How to Cite
Liang, R., Ustinova, E. E., Patnam, R., Fraser, M. O., Gutkin, D. W. and Pezzone, M. A. (2007), Enhanced expression of mast cell growth factor and mast cell activation in the bladder following the resolution of trinitrobenzenesulfonic acid (TNBS) colitis in female rats. Neurourol. Urodyn., 26: 887–893. doi: 10.1002/nau.20410
- Issue published online: 19 SEP 2007
- Article first published online: 23 MAR 2007
- Manuscript Accepted: 8 JAN 2007
- Manuscript Received: 2 OCT 2006
- National Institutes of Health. Grant Numbers: DK02488, DK066658
- neurogenic cystitis;
- stem cell factor
Chronic pelvic pain disorders often overlap. We have shown that acute colonic irritation can produce acute irritative micturition patterns and acutely sensitize bladder afferent responses to mechanical and chemical stimuli. We hypothesize that with time, colonic irritation can lead to neurogenic changes in the bladder and the development of chronic bladder sensitization.
Micturition patterns were measured in rats 60–90 days after the induction of trinitrobenzenesulfonic acid (TNBS) colitis in the resolution phase of this model. Total and activated mast cells (MCs) were quantified in the bladder, while mRNA levels of stem cell factor (SCF; a.k.a. MC growth factor) and nerve growth factor (NGF; a MC and nociceptive C-fiber stimulator) were quantified in the bladder and L6-S1 dorsal root ganglia (DRG).
Following intra-rectal TNBS, voiding volume was reduced (P < 0.005), while voiding frequency was increased (P < 0.05), both by ∼50%. Furthermore, both the percentage and density of activated bladder MCs were significantly elevated (P < 0.05), although total MC counts were not statistically increased. At the molecular level, urinary bladder SCF expression increased twofold (P < 0.005), as did NGF (P < 0.01), while L6-S1 DRG levels were not significantly elevated.
Chronic cystitis in the rat as evidenced by changes in micturition patterns and the recruitment of activated MCs can occur during the resolution phase of TNBS colitis. These changes, of which MCs may play an important role, appear to be maintained over time and may occur via stimulation of convergent pelvic afferent input resulting in the upregulation of neurotrophic factors in the target organ. Neurourol. Urodynam. 26:887–893, 2007. © 2007 Wiley-Liss, Inc.