Conflicts of interest: none.
Original Basic Science Article
Version of Record online: 12 MAR 2009
Copyright © 2009 Wiley-Liss, Inc.
Neurourology and Urodynamics
Volume 28, Issue 6, pages 529–534, August 2009
How to Cite
Imamura, T., Ishizuka, O., Zhong, C., Ogawa, T., Nakayama, T., Kurizaki, Y., Tanabe, T., Nishizawa, O. and Andersson, K.-E. (2009), An extract (THC-002) of Ba-Wei-Die-Huang-Wan inhibits expression of tachykinins, and P2X3 and TRPV1 receptors, and inhibits ATP-induced detrusor overactivity in spontaneously hypertensive rats. Neurourol. Urodyn., 28: 529–534. doi: 10.1002/nau.20702
Lori Birder led the review process.
- Issue online: 9 JUL 2009
- Version of Record online: 12 MAR 2009
- Manuscript Accepted: 30 DEC 2008
- Manuscript Received: 4 NOV 2008
- detrusor overactivity;
- P2X3 receptor;
- TRPV1 receptor;
- urinary bladder
To investigate possible mechanisms of action of THC-002 (HARNCARE®), a galenical produced from the traditional Chinese herbal mixture Ba-Wei-Die-Huang-Wan, which has been reported to improve lower urinary tract symptoms (LUTS) in patients.
Forty-five female SHRs were randomly separated into three groups. Two groups were given 20 ml physiological saline solution (PSS) per kg-body weight orally daily for 1 week. An hour after the administration of PSS, one of the groups received 20 mg THC-002 per kg body weight, and the other a similar volume of THC-002-free saline. The third group received no treatments. The bladders were analyzed by real time RT-PCR (n = 6) and immunohistochemistry (n = 3) for the expression of tachykinins and P2X3 and TRPV1 receptors. Cystometric investigation (n = 6) was conducted after intravesical instillation of saline followed by 5 mg/ml ATP solution.
Treatment with PSS caused and upregulation of tachykinins and P2X3 and TRPV1 receptors, which was prevented in the group treated with THC-002. In the normal (non-treated) and non-THC-002-treated SHRs, instillation of the ATP solution decreased voiding interval, micturition volume, and bladder capacity compared to the instillation of saline. However, in the THC-002-treated SHRs, ATP instillation had no effect.
In SHRs, THC-002 reduced the bladder expression of tachykinins and P2X3 and TRPV1 receptors, and inhibited ATP-induced detrusor overactivity. These effects may explain part of its beneficial effects on LUTS. Neurourol. Urodyn. 28:529–534, 2009. © 2009 Wiley-Liss, Inc.