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Development of bladder dysfunction in a rat model of dopaminergic brain lesion

Authors

  • Roberto Soler,

    1. Wake Forest Institute for Regenerative Medicine, Wake Forest University, Winston Salem, North Carolina
    2. Division of Urology, Federal University of São Paulo, São Paulo, Brazil
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  • Claudius Füllhase,

    1. Wake Forest Institute for Regenerative Medicine, Wake Forest University, Winston Salem, North Carolina
    2. Department of Urology, University Hospital Grosshadern, LMU Munich, Germany
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  • Cesar Santos,

    1. Wake Forest Institute for Regenerative Medicine, Wake Forest University, Winston Salem, North Carolina
    2. Department of Neurology, Wake Forest University School of Medicine, Winston Salem, North Carolina
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  • Karl-Erik Andersson

    Corresponding author
    1. Wake Forest Institute for Regenerative Medicine, Wake Forest University, Winston Salem, North Carolina
    • Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Medical Center Boulevard, Winston Salem, NC 27157.
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  • Conflict of interest: none.

  • Lori Birder led the review process.

Abstract

Aims

Parkinson's disease (PD) is one of the most common neurological disorders causing lower urinary tract dysfunction. We evaluated the temporal development of bladder dysfunction in rat PD model where urodynamic changes were induced by unilateral injection of 6-hydroxydopamine (6-OHDA) into the medial forebrain bundle (MFB).

Methods

Female Sprague–Dawley rats underwent a unilateral stereotaxic injection of 6-OHDA or vehicle (sham group) into the MFB. Cystometry was performed in conscious animals at 3, 14, and 28 days after the injury. Aged-matched unlesioned rats were used as healthy controls.

Results

Three days after lesion 6-OHDA rats showed higher threshold (TP), maximum pressures (MP), and spontaneous activity (SA) compared to healthy controls. Sham animals exhibited higher TP. After 14 days 6-OHDA rats had also higher micturition frequency, decreased bladder capacity, micturition volume and bladder compliance (Bcom) compared to sham and healthy controls. Sham animals showed lower Bcom and higher MP and SA. After 28 days, 6-OHDA rats exhibited the same changes as those in 14 days, while sham-operated animals showed parameters similar to those in healthy controls.

Conclusions

These findings suggest that 6-OHDA lesion of the MFB causes bladder dysfunction already after 3 days. A pattern of detrusor overactivity was more clearly defined 14 days after the injection and persisted for 28 days. Cystometry may be a useful tool to study the pathophysiology of bladder dysfunction in PD, and urodynamic parameters may possibly be used to evaluate the effects of therapeutic interventions. Neurourol. Urodyn. 30:188–193, 2011. © 2010 Wiley-Liss, Inc.

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