Conflicts of interest: De Wacter-Fellowship travel: Astellas. Wyndaele- Consultant: Pfizer, Eli Lilly, Novartis; Honoraria/speaker: Astellas, Pfizer, Novartis, Eli Lilly, Ismar Healthcare, Coloplast; Clinical trial participant: Astellas, Pfizer, GSK, Novartis, Esteril, Allergan, Schwarz; Fellowship/grants: Astellas, Aventis, Zambou, Glaxo, Innovex, PPD, Synthelabs, Parexel, Bayer.
Original Basic Science Article
Article first published online: 21 SEP 2010
Copyright © 2010 Wiley-Liss, Inc.
Neurourology and Urodynamics
Volume 30, Issue 1, pages 158–162, January 2011
How to Cite
De Bock, F., De Wachter, S. and Wyndaele, J.-J. (2011), Exploring the mechanisms of intravesical electrical stimulation in the in vitro rat whole bladder after treatment with atropine, α,β-methylATP and tetrodotoxin. Neurourol. Urodyn., 30: 158–162. doi: 10.1002/nau.20949
Karl-Erik Andersson led the review process.
- Issue published online: 22 DEC 2010
- Article first published online: 21 SEP 2010
- Manuscript Accepted: 22 APR 2010
- Manuscript Received: 2 FEB 2010
- Universitair Ziekenhuis Antwerpen (UZA)
- intravesical electrical stimulation;
- in vitro;
- whole bladder;
In a previous study, we showed that the working mechanism of intravesical electrical stimulation (IVES) is probably mainly nerve mediated. But even after bladder decentralization, IVES can induce detrusor contraction. This study explores the effect of IVES in decentralized bladders and the importance of receptors in the bladder wall for a response on IVES.
IVES (10 Hz square wave pulses, 20 msec pulse duration, 6 mA) was used in the bladder of 16 female Sprague–Dawley rats. After repeating IVES after consecutive bilateral bladder nerves section (L6-roots, pelvic nerves, and major pelvic ganglion (MPG)), the bladders were mounted in a tissue bath. IVES was performed in the control (n = 16), after administration of tetrodotoxin (TTX) (n = 6), after atropine and atropine with α,β-methylATP (n = 6), and after α,β-methylATP and α,β-methylATP with atropine (n = 4). The IVES-induced pressure rise (ΔP) was recorded.
Maximum ΔP (maxΔP) after transection of the MPG was significantly lower than after pelvic nerves transection. Treatment with TTX and with α,β-methylATP plus atropine abolished ΔP. Atropine alone gave an insignificant decrease of maxΔP. Treatment with α,β-methylATP alone reduced maxΔP significantly.
IVES can evoke contractions in a decentralized bladder. IVES-induced contractions are not a result of direct muscle stimulation, but are nerve mediated, involving intramural innervation and several parts of the bladder innervation. IVES-evoked contraction can be divided in a, contraction duration determining, cholinergic part and a, contraction strength determining, purinergic part. The peripheral innervation could play a role in IVES treatment in patients with interrupted central reflex pathway. Neurourol. Urodynam. 30:158–162, 2011. © 2010 Wiley-Liss, Inc.