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Pulsed magnetic stimulation with a high-frequency continuous magnetic stimulator (SMN-X) does not exert an adverse effect on genital organs and the estrous cycle in female Iar:Wistar-Imamichi rats

Authors


  • Conflict of interest: none.

  • Lori Birder led the review process.

Abstract

Aims

To histopathologically verify whether high-frequency continuous stimulation exerts adverse effects on genital organs (uterus and ovaries) and the estrous cycle in rats through comparison of 3 groups.

Methods

The device studied was a high-frequency continuous magnetic stimulator (SMN-X, Nihon Kohden, Tokyo). Thirteen female Iar:Wistar-Imamichi rats (SPF) were randomly divided into 3 groups: active treatment (MS) group (n = 7), sham treatment group, where rats were placed on a table 1 m away from the stimulator so that they could hear the sounds of the stimulator (n = 3) and control (no treatment) group (n = 3). The MS group underwent thirty-six 25-minute sessions of pulsed magnetic stimulation at the maximum output level (560 mT peak) (once/day, 5 days/week). At the end of the study period, the uterus and ovaries were removed for histological examinations. Estradiol and progesterone levels were assayed and the estrous cycle, bodyweight gain and daily behaviors were recorded.

Results

From results of histopathology and endocrinology, assays of the estrous cycle, and recordings of bodyweight gain and organ weight, no adverse effects of long-term pulsed magnetic stimulation were noted. There were no significant intergroup differences with regard to any item evaluated.

Conclusion

In the study setting, SMN-X did not exert any adverse effect on the uterus, ovaries, estrous cycle and hormone levels as well as blood cell counts, bodyweight gain and daily behavior in female SPF rats. The present results may be a step forward in the discussion of the safety of SMN-X for clinical use in humans. Neurourol. Urodynam. 30: 1675–1680, 2011. © 2011 Wiley Periodicals, Inc.

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