Heinz Koelbl led the review process.
Original Basic Science Article
Article first published online: 28 OCT 2011
Copyright © 2011 Wiley Periodicals, Inc.
Neurourology and Urodynamics
Volume 31, Issue 1, pages 109–114, January 2012
How to Cite
Bortolini, M. A.T., Shynlova, O., Drutz, H. P., Castro, R. A., Girão, M. J.B.C., Lye, S. and Alarab, M. (2012), Expression of genes encoding smooth muscle contractile proteins in vaginal tissue of women with and without pelvic organ prolapse. Neurourol. Urodyn., 31: 109–114. doi: 10.1002/nau.21175
Conflict of interest: The authors declare no conflict of interest.
Data presented at the 35th Annual Meeting of the International Urogynecologic Association (IUGA), in August 25, 2010, Toronto, ON, CA.
- Issue published online: 23 JAN 2012
- Article first published online: 28 OCT 2011
- Manuscript Accepted: 22 MAY 2011
- Manuscript Received: 25 FEB 2011
- smooth muscle;
We hypothesize that the expression of genes encoding vaginal smooth muscle (SM) contractile proteins is altered in patients with pelvic organ prolapse (POP) and is influenced by age and menopausal status. We aim to analyze the expression of SM-myosin heavy chain (MHY11), caldesmon (CALD1), SM gamma-actin (ACTG2), and tropomyosin (TPM1), in premenopausal and postmenopausal women with advanced POP and asymptomatic controls.
During total hysterectomy we collected anterior vaginal wall biopsy samples from 55 women, 37 premenopausal (23 patients and 14 controls), and 18 postmenopausal women (13 patients and 5 controls). Total mRNA from the tissues was quantified by real-time RT-PCR.
MHY11 gene expression was down-regulated in premenopausal POP patients compared to premenopausal controls (fivefold, P = 0.002). In the postmenopausal groups, we observed a sixfold increase in the CALD1 gene expression in POP patients compared to asymptomatic controls (P = 0.03). The gene expression of CALD1, ACTG2, and TPM1 was significantly down-regulated in vaginal tissue of healthy women after menopause (P < 0.05).
Dysregulation of the vaginal SM content in POP patients involves alteration of different cellular pathways according to age and menopausal status. Neurourol. Urodynam. 31:109–114, 2012. © 2011 Wiley Periodicals, Inc.