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Expression of genes encoding smooth muscle contractile proteins in vaginal tissue of women with and without pelvic organ prolapse§

Authors

  • Maria A.T. Bortolini,

    Corresponding author
    1. Division of Urogynecology and Reconstructive Pelvic Surgery, Department of Obstetrics & Gynecology, Mount Sinai Hospital, University of Toronto, Toronto, Ontario, Canada
    2. Division of Urogynecology and Reconstructive Pelvic Surgery, Department of Gynecology, Federal University of São Paulo, São Paulo, Brazil
    • Division of Urogynecology and Reconstructive Pelvic Surgery, Mount Sinai Hospital, 700 University Avenue, Suite 3149, Toronto, ON, Canada M5G1Z5.
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  • Oksana Shynlova,

    1. Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
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  • Harold P. Drutz,

    1. Division of Urogynecology and Reconstructive Pelvic Surgery, Department of Obstetrics & Gynecology, Mount Sinai Hospital, University of Toronto, Toronto, Ontario, Canada
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  • Rodrigo A. Castro,

    1. Division of Urogynecology and Reconstructive Pelvic Surgery, Department of Gynecology, Federal University of São Paulo, São Paulo, Brazil
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  • Manoel J.B.C. Girão,

    1. Division of Urogynecology and Reconstructive Pelvic Surgery, Department of Gynecology, Federal University of São Paulo, São Paulo, Brazil
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  • Stephen Lye,

    1. Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
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  • May Alarab

    1. Division of Urogynecology and Reconstructive Pelvic Surgery, Department of Obstetrics & Gynecology, Mount Sinai Hospital, University of Toronto, Toronto, Ontario, Canada
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  • Heinz Koelbl led the review process.

  • Conflict of interest: The authors declare no conflict of interest.

  • §

    Data presented at the 35th Annual Meeting of the International Urogynecologic Association (IUGA), in August 25, 2010, Toronto, ON, CA.

Abstract

Aims

We hypothesize that the expression of genes encoding vaginal smooth muscle (SM) contractile proteins is altered in patients with pelvic organ prolapse (POP) and is influenced by age and menopausal status. We aim to analyze the expression of SM-myosin heavy chain (MHY11), caldesmon (CALD1), SM gamma-actin (ACTG2), and tropomyosin (TPM1), in premenopausal and postmenopausal women with advanced POP and asymptomatic controls.

Methods

During total hysterectomy we collected anterior vaginal wall biopsy samples from 55 women, 37 premenopausal (23 patients and 14 controls), and 18 postmenopausal women (13 patients and 5 controls). Total mRNA from the tissues was quantified by real-time RT-PCR.

Results

MHY11 gene expression was down-regulated in premenopausal POP patients compared to premenopausal controls (fivefold, P = 0.002). In the postmenopausal groups, we observed a sixfold increase in the CALD1 gene expression in POP patients compared to asymptomatic controls (P = 0.03). The gene expression of CALD1, ACTG2, and TPM1 was significantly down-regulated in vaginal tissue of healthy women after menopause (P < 0.05).

Conclusion

Dysregulation of the vaginal SM content in POP patients involves alteration of different cellular pathways according to age and menopausal status. Neurourol. Urodynam. 31:109–114, 2012. © 2011 Wiley Periodicals, Inc.

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