Lori Birder led the review process.
Original Basic Science Article
Article first published online: 8 AUG 2011
Copyright © 2011 Wiley Periodicals, Inc.
Neurourology and Urodynamics
Volume 31, Issue 1, pages 162–167, January 2012
How to Cite
Chang, H. H. and Havton, L. A. (2012), Systemic administration of fluorogold for anatomical pre-labeling of autonomic and motor neurons in the rat spinal cord compromises urodynamic recordings in acute but not long-term studies. Neurourol. Urodyn., 31: 162–167. doi: 10.1002/nau.21184
Conflict of interest: none.
- Issue published online: 23 JAN 2012
- Article first published online: 8 AUG 2011
- Manuscript Accepted: 13 JUN 2011
- Manuscript Received: 13 MAY 2011
- National Institutes of Health RO1 NS42719
- Dr. Miriam and Sheldon G. Adelson Medical Research Foundation
- external urethral sphincter;
- pre-ganglionic parasympathetic neurons
The use of anatomical tracer injections into peripheral tissues for retrograde labeling of spinal cord neurons may compromise physiological experiments in combined functional and morphological studies.
We investigated whether a systemic injection of a retrogradely transported tracer, fluorogold (FG), may provide an alternative to direct injections into end organs for combined anatomical and physiological studies of the lower urinary tract. Urodynamic studies including cystometrogram recordings and external urethral sphincter electromyography were used as functional outcome measures.
Pre-labeling of spinal cord neurons by intraperitoneal (i.p.) administration of FG resulted in a transient decrease in voiding efficiency, increase in resting pressure as well as increase in bladder size and weight at 5–7 days after the tracer administration. In contrast, there were no urodynamic or end-organ effects detected at 6–8 weeks after the i.p. injection of FG.
We suggest that pre-labeling of spinal autonomic and motor neurons using i.p. administration of FG may be a useful tool when combining anatomical and functional outcome measures in long-term but not acute studies. Neurourol. Urodynam. 31:162–167, 2012. © 2011 Wiley Periodicals, Inc.