Lori Birder led the peer-review process as the Associate Editor responsible for the paper.
Original Basic Science Article
Article first published online: 21 SEP 2012
Copyright © 2012 Wiley Periodicals, Inc.
Neurourology and Urodynamics
Volume 32, Issue 5, pages 500–504, June 2013
How to Cite
Lei, Z., Ishizuka, O., Imamura, T., Noguchi, W., Yamagishi, T., Yokoyama, H., Kurizaki, Y., Sudha, G. S., Hosoda, T., Nishizawa, O. and Andersson, K.-E. (2013), Functional roles of transient receptor potential melastatin 8 (TRPM8) channels in the cold stress-induced detrusor overactivity pathways in conscious rats. Neurourol. Urodyn., 32: 500–504. doi: 10.1002/nau.22325
Conflict of interest: none.
- Issue published online: 18 JUN 2013
- Article first published online: 21 SEP 2012
- Manuscript Accepted: 31 AUG 2012
- Manuscript Received: 10 JUL 2012
- low temperature;
- TRPM8 channel antagonist;
We determined if transient receptor potential melastatin 8 (TRPM8) channels are involved in the detrusor overactivity induced by menthol, or exposure to low temperature (LT).
Two days prior to cystometric investigation, the bladders of 10-week-old Sprague–Dawley rats were cannulated to measure bladder pressure. After a 20 min baseline cystometry period, the TRPM8 channel antagonist, N-(4-tert-butylphenyl)-4-(3-chloropyridin-2-yl)piperazine-1-carboxamide (BCTC), or vehicle, was administered through a jugular vein catheter (n = 6). A 90% menthol solution was sprayed onto bare leg skin once every 5 min for 20 min, and then cystometric measurements were repeated. After a 30-min recovery period, the rats were intravenously administered 0.1 µmol/kg BCTC. Five minutes later, they were again sprayed and cystometry recorded. In separate experiments, cannulated rats were intravenously administered 0.001, 0.01, or 0.1 µmol/kg BCTC (n = 6 each dose). Five minutes later, they were exposed to LT (4 ± 2°C) for 20 min of cystometry.
Menthol spray decreased voiding interval, micturition volume, and bladder capacity in the BCTC-free rats. However after BCTC administration, these effects were prevented. Exposure to LT elicited detrusor overactivity that caused decreased voiding interval, micturition volume, and bladder capacity. However, at 0.01 and 0.1 µmol/kg, BCTC inhibited this cold stress-induced detrusor overactivity.
Since the TRPM8 channel agonist, BCTC, inhibited detrusor overactivity in rats sprayed with the TRPM8 channel agonist, menthol, and the drug also inhibited cold stress-induced detrusor overactivity, we conclude that TRPM8 channels mediate, at least partially, detrusor overactivity elicited by exposure to LT. Neurourol. Urodynam. 32: 500–504, 2013. © 2012 Wiley Periodicals, Inc.