Christopher Chapple led the peer-review process as the Associate Editor responsible for the paper.
Original Basic Science Article
Article first published online: 5 NOV 2012
Copyright © 2012 Wiley Periodicals, Inc.
Neurourology and Urodynamics
Volume 32, Issue 5, pages 493–499, June 2013
How to Cite
Ha, U.-S., Bae, W.-J., Kim, S.-J., Yoon, B.-I., Jang, H., Hong, S.-H., Lee, J.-Y., Hwang, S.-Y. and Kim, S.-W. (2013), Protective effect of cyanidin-3-O-β-D-glucopyranoside fraction from mulberry fruit pigment against oxidative damage in streptozotocin-induced diabetic rat bladder. Neurourol. Urodyn., 32: 493–499. doi: 10.1002/nau.22334
Conflict of interest: none.
- Issue published online: 18 JUN 2013
- Article first published online: 5 NOV 2012
- Manuscript Accepted: 18 SEP 2012
- Manuscript Received: 7 MAR 2012
- cyaniding 3-O-β-D-glucopyranoside;
- diabetes mellitus;
- urinary bladder
To determine whether cyanidin-3-O-β-D-glucopyranoside (C3G) fraction from mulberry fruit pigment has protective effects against bladder dysfunction on streptozotocin-induced diabetic rats
Sprague–Dawley rats were divided into three groups (n = 12 in each): normal, diabetes (DM), and DM treated with C3G fraction (DM + C3G). The DM and DM + C3G groups received a single injection of streptozotocin (50 mg/kg) intraperitoneally. Four weeks after the induction of diabetes, the DM + C3G group was treated with daily oral C3G (10 mg/kg) dissolved in water, for 8 weeks. After 12 weeks of streptozotocin injections, rats in each group underwent cystometrography and bladders were used for evaluation of apoptosis and oxidative stress.
The DM group showed a markedly lower maximal intravesical pressure than that observed in the control group, whereas rats in the DM + C3G group showed improved maximum intravesical pressure associated with minimization of apoptosis, and increased levels of Akt and Bad phosphorylation, implying inhibition of pro-apoptotic stimuli. The level of 8-hydroxy-2-deoxyguanosine, a marker of oxidative stress, was significantly greater in the DM group compared to the control group and it was significantly reduced in the C3G treated group. Immunoblotting revealed a significant decrease in the levels of the superoxide dismutase protein and nerve growth factor in the DM group compared with the control group; however, these proteins were upregulated in the DM + C3G group compared with the DM group.
The study is the first to suggest that C3G fraction have a potency to protect the bladder under conditions of diabetes-induced oxidative stress. Neurourol. Urodynam. 32: 493–499, 2013. © 2012 Wiley Periodicals, Inc.