Karl-Erik Andersson led the peer-review process as the Associate Editor responsible for the paper.
Original Basic Science Article
Article first published online: 9 NOV 2012
Copyright © 2012 Wiley Periodicals, Inc.
Neurourology and Urodynamics
Volume 32, Issue 7, pages 1031–1037, September 2013
How to Cite
Kawai, Y., Oka, M., Kyotani, J., Oyama, T., Matsumoto, S. and Kakizaki, H. (2013), Effect of the phytotherapeutic agent eviprostat on the bladder in a rat model of bladder overdistension/emptying. Neurourol. Urodyn., 32: 1031–1037. doi: 10.1002/nau.22350
Conflict of interest: none.
- Issue published online: 12 AUG 2013
- Article first published online: 9 NOV 2012
- Manuscript Accepted: 12 OCT 2012
- Manuscript Received: 9 JUL 2012
- benign prostatic hyperplasia;
- bladder blood flow;
- bladder function;
- oxidative stress;
- proinflammatory cytokines
Ischemia–reperfusion injury is an important factor in the development of lower urinary tract symptoms (LUTS) that is partly mediated by the generation of free radicals. We investigate the effect of the phytotherapeutic agent Eviprostat, a treatment for benign prostatic hyperplasia (BPH) that has antioxidant and anti-inflammatory activity, on urinary bladder blood flow (BBF), and function in a rat model of bladder overdistension and emptying (OE).
For 8 days before surgery, OE rats received daily oral Eviprostat (36 mg/kg/day) or vehicle, while sham-operated animals received vehicle. The bladder was distended by infusion of saline over a period of 2 hr (overdistension) and then emptied. After 24 hr, BBF was measured with a laser speckle blood flow imager. The oxidative-stress marker malondialdehyde (MDA), proinflammatory cytokines, and myeloperoxidase were determined in the isolated bladder, and histological analysis was performed. Functional contractile responses of bladder strips to electrical field stimulation, carbachol, and KCl were measured.
Twenty-four hours after bladder OE, a significant decrease in BBF and significant increases in bladder weight, malondialdehyde, proinflammatory cytokines, and myeloperoxidase were observed. Eviprostat almost completely prevented these changes. Histological analysis of the bladder of OE rats showed hemorrhage, accumulation of leukocytes, desquamation of epithelium, and edema, and Eviprostat suppressed these changes. The reduction in functional contractile forces in the bladder of OE rats was also prevented by Eviprostat.
Eviprostat-mediated suppression of increased bladder oxidative stress and inflammation caused by bladder OE may contribute to the improvement of BBF and bladder function by Eviprostat. Neurourol. Urodynam. 32: 1031–1037, 2013. © 2012 Wiley Periodicals, Inc.