Karl-Erik Andersson led the peer-review process as the Associate Editor responsible for the paper.
Original Basic Science Article
Article first published online: 20 NOV 2012
Copyright © 2012 Wiley Periodicals, Inc.
Neurourology and Urodynamics
Volume 32, Issue 7, pages 1026–1030, September 2013
How to Cite
Honda, M., Yoshimura, N., Hikita, K., Hinata, N., Muraoka, K., Saito, M., Chancellor, M. B. and Takenaka, A. (2013), Supraspinal and spinal effects of L-trans-PDC, an inhibitor of glutamate transporter, on the micturition reflex in rats. Neurourol. Urodyn., 32: 1026–1030. doi: 10.1002/nau.22351
Conflict of interest: None.
- Issue published online: 12 AUG 2013
- Article first published online: 20 NOV 2012
- Manuscript Accepted: 15 OCT 2012
- Manuscript Received: 5 AUG 2012
- NIH. Grant Numbers: DK057267, DK068557
- PVA. Grant Number: 2793
- glutamate transporter;
Glutamate is a major excitatory transmitter in the central nervous system, controlling lower urinary tract function. Five types of glutamate transporters such as GLAST (EAAT1), GLT-1 (EAAT2), EAAC-1 (EAAT3), EAAT4, and EAAT5 have been cloned so far. In the current study we tested whether L-trans-pyrrolidine-2,4-dicarboxylic acid (L-trans-PDC), a non-selective inhibitor of glutamate transporters that increases endogenous glutamate concentration, can affect the micturition reflex in urethane anesthetized rats.
Continuous cystometrograms (CMG, 0.04 ml/min infusion rate) were performed in two groups of urethane-anesthetized rats. A group of 18 rats was used for intrathecal administration of 1–10 µg of L-trans-PDC via an intrathecal catheter. In the second group of 18 rats, 1–10 µg of L-trans-PDC were administered intracerebroventricularly via a catheter inserted into the lateral ventricle. Micturition parameters were recorded and compared before and after drug administration.
Intrathecal administration of L-trans-PDC at 1, 3, and 10 µg (n = 6 per dose) increased intercontraction intervals in dose dependent fashion, but did not affect postvoid residual or basal pressure at any doses tested. Intracerebroventricular administration of L-trans-PDC at 1, 3, and 10 µg (n = 6 per dose) also increased intercontraction intervals in dose dependent fashion, but did not affect postvoid residual or basal pressure at any doses tested.
The current results show that, in urethane-anesthetized rats, suppression of glutamate transporters by L-trans-PDC has an inhibitory effect on the micturition reflex at supraspinal and spinal sites, possibly via activation of glutamate-mediated inhibitory pathways. Neurourol. Urodynam. 32: 1026–1030, 2013. © 2012 Wiley Periodicals, Inc.