Get access

The effects of Ins2(Akita) diabetes and chronic angiotensin II infusion on cystometric properties in mice

Authors

  • Paul C. Dolber,

    Corresponding author
    1. Veterans Affairs Medical Center, Durham, North Carolina
    2. Department of Surgery, Duke University Medical Center, Durham, North Carolina
    3. Department of Pathology, Duke University Medical Center, Durham, North Carolina
    4. Institute for Medical Research, Durham, North Carolina
    • Correspondence to: Dr. Paul C. Dolber, Research Services (151), Veterans Affairs Medical Center, 508 Fulton Street Durham, NC 27705. E-mail: dolber@duke.edu

    Search for more papers by this author
  • Huixia Jin,

    1. Veterans Affairs Medical Center, Durham, North Carolina
    2. Department of Surgery, Duke University Medical Center, Durham, North Carolina
    Search for more papers by this author
  • Rashid Nassar,

    1. Institute for Medical Research, Durham, North Carolina
    Search for more papers by this author
  • Thomas M. Coffman,

    1. Veterans Affairs Medical Center, Durham, North Carolina
    2. Department of Medicine, Duke University Medical Center, Durham, North Carolina
    Search for more papers by this author
  • Susan B. Gurley,

    1. Veterans Affairs Medical Center, Durham, North Carolina
    2. Department of Medicine, Duke University Medical Center, Durham, North Carolina
    Search for more papers by this author
  • Matthew O. Fraser

    1. Veterans Affairs Medical Center, Durham, North Carolina
    2. Department of Surgery, Duke University Medical Center, Durham, North Carolina
    3. Institute for Medical Research, Durham, North Carolina
    Search for more papers by this author

  • Karl-Erik Andersson led the peer-review process as the Associate Editor responsible for the paper.
  • Conflict of interest: none.

Abstract

Aims

Diabetes is associated with both dysfunction of the lower urinary tract (LUT) and overactivity of the renin-angiotensin system (RAS). Although it is well known that the RAS affects normal LUT function, very little is known about RAS effects on the diabetic LUT. Accordingly, we investigated the effects of chronic angiotensin II (AngII) treatment on the LUT in a model of type 1 diabetes.

Methods

Ins2(Akita) diabetic mice (20 weeks old) and their age-matched background controls underwent conscious cystometric evaluation after 4 weeks of chronic AngII treatment (700 ng/kg/min by osmotic pump) or vehicle (saline).

Results

Diabetic mice had compensated LUT function with bladder hypertrophy. Specifically, micturition volume, residual volume, and bladder capacity were all increased, while voiding efficiency and pressure generation were unchanged as bladder mass, contraction duration, and phasic urethral function were increased. AngII significantly increased voiding efficiency and peak voiding pressure and decreased phasic frequency irrespective of diabetic state and, in diabetic but not normoglycemic control mice, significantly decreased residual volume and increased contraction duration and nonphasic contraction duration.

Conclusions

The Ins2(Akita) diabetic mice had compensated LUT function at 20 weeks of age. Even under these conditions, AngII had beneficial effects on LUT function, resulting in increased voiding efficiency. Future studies should therefore be conducted to determine whether AngII can rescue the decompensated LUT function occurring in end-stage diabetic uropathy. Neurourol. Urodynam. 34:72–78, 2015. © 2013 Wiley Periodicals, Inc.

Ancillary