This study was supported by the research grant from Mochida Pharmaceutical CO., LTD. (Tokyo, Japan).
Influence of urethane-anesthesia on the effect of resiniferatoxin treatment on bladder function in rats with spinal cord injury
Article first published online: 28 DEC 2013
© 2013 Wiley Periodicals, Inc.
Neurourology and Urodynamics
Volume 34, Issue 3, pages 274–279, March 2015
How to Cite
Aizawa, N., Ogawa, S., Sugiyama, R., Homma, Y. and Igawa, Y. (2015), Influence of urethane-anesthesia on the effect of resiniferatoxin treatment on bladder function in rats with spinal cord injury. Neurourol. Urodyn., 34: 274–279. doi: 10.1002/nau.22549
Lori Birder led the peer-review process as the Associate Editor responsible for the paper.
- Issue published online: 12 MAR 2015
- Article first published online: 28 DEC 2013
- Manuscript Accepted: 26 NOV 2013
- Manuscript Received: 24 SEP 2013
- Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sport, Science and Technology of the Japanese Government. Grant Numbers: 40159588, 80595257
- Mochida Pharmaceutical CO., LTD., Tokyo, Japan
- spinal cord injury;
- Sprague-Dawley rats;
- urinary bladder
We investigated the effect of resiniferatoxin (RTX)-treatment on cystometric parameters in the spinal cord injury (SCI) rats in both conscious and urethane-anesthetized conditions and evaluated the influence of urethane-anesthesia on the effect of RTX on lower urinary tract (LUT) function in SCI rats.
Female Sprague-Dawley rats were used. SCI was created by transection of the T8-T9 spinal cord. Four weeks after the transection, the animals were placed in a restraint cage for the first cystometric measurements in a conscious state. Secondary cystometric measurements were performed in a conscious condition following the 1 day after RTX-(0.3 mg/kg) or vehicle-subcutaneous injection. Then the animals were injected with urethane (1.5 g/kg, subcutaneously), and cystometric measurements were repeated four times every 1 hr-interval.
After the RTX-treatment in a conscious condition, urinary retention was observed in three out of five animals. In addition, the number of non-voiding contractions (NVCs) significantly decreased although their amplitude did not change significantly. After the urethane-injection, all of the animals treated with RTX developed urinary retention. The amplitude of NVCs significantly decreased, whereas the number of NVCs did not change significantly in the RTX-treated group. No cystometric parameters significantly changed after either vehicle- or urethane-injection in the vehicle-treated group.
The present results indicate that the suppressive effects of RTX on NVCs as well as voiding contractions in SCI rats can be enhanced by urethane-anesthesia. Such suppressive effect of urethane-anesthesia itself should be taken into consideration when we evaluate a drug-effect on LUT function in rats with SCI. Neurourol. Urodynam. 34:274–279, 2015. © 2013 Wiley Periodicals, Inc.