Research Article

Fractionated manganese-enhanced MRI†

  1. Nicholas A. Bock*,
  2. Fernando F. Paiva,
  3. Afonso C. Silva

Article first published online: 18 OCT 2007

DOI: 10.1002/nbm.1211

Issue

NMR in Biomedicine

NMR in Biomedicine

Volume 21, Issue 5, pages 473–478, June 2008

Additional Information

How to Cite

Bock, N. A., Paiva, F. F. and Silva, A. C. (2008), Fractionated manganese-enhanced MRI. NMR Biomed., 21: 473–478. doi: 10.1002/nbm.1211

Author Information

  1. Cerebral Microcirculation Unit/Laboratory of Functional and Molecular Imaging/National Institute of Neurological Disorders and Stroke/National Institutes of Health, Bethesda, MD,USA

*Cerebral Microcirculation Unit/Laboratory of Functional and Molecular Imaging/National Institute of Neurological Disorders and Stroke/National Institutes of Health, 10 Center Drive, Building 10, Room BD109, Bethesda, MD 20892-1065, USA.

  1. †

    This article is a U.S. Government work and is in the public domain in the U.S.A.

Publication History

  1. Issue published online: 28 MAY 2008
  2. Article first published online: 18 OCT 2007
  3. Manuscript Revised: 26 JUN 2007
  4. Manuscript Accepted: 26 JUN 2007
  5. Manuscript Received: 12 JUN 2007

Funded by

  • Intramural Research Program of the NIH, NINDS

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

Get PDF (212K)

Keywords:

  • rodent;
  • central nervous system;
  • MRI;
  • manganese;
  • contrast agent

Abstract

We investigated the use of manganese-enhanced MRI (MEMRI) with fractionated doses as a way to retain the unique properties of manganese as a neuronal contrast agent while lessening its toxic effects in animals. First, we followed the signal enhancement on T1-weighted images of the brains of rats receiving 30 mg/kg fractions of MnCl2 · 4H2O every 48 h and found that the signal increased in regions with consecutive fractionated doses and ultimately saturated. Second, we used T1 mapping to test whether the amount of MRI-visible manganese that accumulated depended on the concentration of manganese in the fractions. For a fixed cumulative dose of 180 mg/kg MnCl2 · 4H2O, increasing fraction doses of 6 × 30 mg/kg, 3 × 60 mg/kg, 2 × 90 mg/kg and 1 × 180 mg/kg produced progressively shorter T1 values. The adverse systemic health effects, including complications at the injection site and poor animal well-being, also rose with the fraction dose. Thus, fractionated MEMRI can be used to balance the properties of manganese as a contrast agent in animals against its toxic effects. Published in 2007 by John Wiley & Sons, Ltd.

Get PDF (212K)