This article was published online on 19/11/08. Errors were subsequently identified. This notice is included in the online and print versions to indicate that both have been corrected 12/01/09.
Metabolite profiling of the intraerythrocytic malaria parasite Plasmodium falciparum by 1H NMR spectroscopy†
Article first published online: 19 NOV 2008
Copyright © 2009 John Wiley & Sons, Ltd.
NMR in Biomedicine
Volume 22, Issue 3, pages 292–302, April 2009
How to Cite
Teng, R., Junankar, P. R., Bubb, W. A., Rae, C., Mercier, P. and Kirk, K. (2009), Metabolite profiling of the intraerythrocytic malaria parasite Plasmodium falciparum by 1H NMR spectroscopy. NMR Biomed., 22: 292–302. doi: 10.1002/nbm.1323
- Issue published online: 3 MAR 2009
- Article first published online: 19 NOV 2008
- Manuscript Accepted: 17 JUL 2008
- Manuscript Revised: 19 JUN 2008
- Manuscript Received: 4 FEB 2007
- Australian National Health and Medical Research Council. Grant Number: 316933
- 1H NMR;
- metabolite profiling;
- intracellular concentrations
NMR spectroscopy was used to identify and quantify compounds in extracts prepared from mature trophozoite-stage Plasmodium falciparum parasites isolated by saponin-permeabilisation of the host erythrocyte. One-dimensional 1H NMR spectroscopy and four two-dimensional NMR techniques were used to identify more than 50 metabolites. The intracellular concentrations of over 40 metabolites were estimated from the 1H NMR spectra of extracts prepared by four extraction methods: perchloric acid, methanol/water, methanol/chloroform/water, and methanol alone. The metabolites quantified included: the majority of the biological α-amino acids; 4-aminobutyric acid; mono-, di- and tri-carboxylic acids; nucleotides; polyamines; myo-inositol; and phosphocholine and phosphoethanolamine. The parasites also contained a significant concentration (up to 12 mM) of the exogenous buffering agent, HEPES. Although the metabolite profiles obtained with each extraction method were broadly similar, perchloric acid was found to have significant advantages over the other extraction media. Copyright © 2009 John Wiley & Sons, Ltd.