Research Article

Manganese-enhanced MRI visualizes V1 in the non-human primate visual cortex†

  1. Nicholas A. Bock*,
  2. Ara Kocharyan,
  3. Afonso C. Silva

Article first published online: 25 MAR 2009

DOI: 10.1002/nbm.1384

Issue

NMR in Biomedicine

NMR in Biomedicine

Volume 22, Issue 7, pages 730–736, August 2009

Additional Information

How to Cite

Bock, N. A., Kocharyan, A. and Silva, A. C. (2009), Manganese-enhanced MRI visualizes V1 in the non-human primate visual cortex. NMR Biomed., 22: 730–736. doi: 10.1002/nbm.1384

Author Information

  1. Cerebral Microcirculation Unit, Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, National Institutes of Health 10 Center Drive, Building 10, Room BD109 Bethesda, MD 20892-1065, USA

*Cerebral Microcirculation Unit, Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, National Institutes of Health 10 Center Drive, Building 10, Room BD109 Bethesda, MD 20892-1065, USA.

  1. †

    This article is a US Government work and is in the public domain in the USA.

Publication History

  1. Issue published online: 7 AUG 2009
  2. Article first published online: 25 MAR 2009
  3. Manuscript Revised: 4 FEB 2009
  4. Manuscript Accepted: 4 FEB 2009
  5. Manuscript Received: 26 AUG 2008

Funded by

  • Intramural Research Program of the NIH, NINDS

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Keywords:

  • manganese-enhanced MRI (MEMRI);
  • visual cortex;
  • marmoset;
  • non-human primate;
  • Gennari stripe;
  • cytochrome oxidase

Abstract

MRI at 7 Tesla has been used to investigate the accumulation of manganese in the occipital cortex of common marmoset monkeys (Callithrix jacchus) after administering four fractionated injections of 30 mg/kg MnCl2 · 4H2O in the tail vein. We found a statistically significant decrease in T1 in the primary (V1) and secondary (V2) areas of the visual cortex caused by an accumulation of manganese. The larger T1 shortening in V1 (ΔT1 = 640 ms) relative to V2 (ΔT1 = 490 ms) allowed us to robustly detect the V1/V2 border in vivo using heavily T1-weighted MRI. Furthermore, the dorso-medial (DM) and middle-temporal (MT) areas of the visual pathway could be identified by their T1-weighted enhancement. We showed by comparison to histological sections stained for cytochrome oxidase (CO) activity that the extent of V1 is accurately identified throughout the visual cortex by manganese-enhanced MRI (MEMRI). This provides a means of visualizing functional cortical regions in vivo and could be used in longitudinal studies of phenomena such as cortical plasticity, and for non-destructive localization of cortical regions to guide in the implementation of functional techniques. Published in 2009 by John Wiley & Sons, Ltd.

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