Optimization of magnetosonoporation for stem cell labeling

Authors

  • Daohai Xie,

    1. Image-Guided Bio-Molecular Interventions Section, Department of Radiology, University of Washington School of Medicine, Seattle, USA
    2. Department of Radiology, Suzhou University School of Medicine, Jiangsu, China
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  • Bensheng Qiu,

    Corresponding author
    1. Image-Guided Bio-Molecular Interventions Section, Department of Radiology, University of Washington School of Medicine, Seattle, USA
    • Image-Guided Bio-Molecular Interventions Section, Department of Radiology, University of Washington School of Medicine, 1959 NE Pacific Street, HSC AA-036, Box 357115, Seattle, WA98195, USA.
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  • Piotr Walczak,

    1. Division of MR Research, Russell H. Morgan Department of Radiology, The Johns Hopkins University School of Medicine, Baltimore, USA
    2. Cellular Imaging Section, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, USA
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  • Xubin Li,

    1. Image-Guided Bio-Molecular Interventions Section, Department of Radiology, University of Washington School of Medicine, Seattle, USA
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  • Jesus Ruiz-Cabello,

    1. Division of MR Research, Russell H. Morgan Department of Radiology, The Johns Hopkins University School of Medicine, Baltimore, USA
    2. Cellular Imaging Section, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, USA
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  • Satoshi Minoshima,

    1. Image-Guided Bio-Molecular Interventions Section, Department of Radiology, University of Washington School of Medicine, Seattle, USA
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  • Jeff W. M. Bulte,

    1. Division of MR Research, Russell H. Morgan Department of Radiology, The Johns Hopkins University School of Medicine, Baltimore, USA
    2. Department of Biomedical Engineering, The Johns Hopkins University School of Medicine, Baltimore, USA
    3. Department of Chemical and Biomolecular Engineering; The Johns Hopkins University School of Medicine, Baltimore, USA
    4. Cellular Imaging Section, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, USA
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  • Xiaoming Yang

    Corresponding author
    1. Image-Guided Bio-Molecular Interventions Section, Department of Radiology, University of Washington School of Medicine, Seattle, USA
    • Image-Guided Bio-Molecular Interventions Section, Department of Radiology, University of Washington School of Medicine, 1959 NE Pacific Street, HSC AA-036, Box 357115, Seattle, WA98195, USA.
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Abstract

Recent advances in magnetic cell labeling have taken place with the development of a magnetosonoporation (MSP) technique. The aim of this study was to optimize the MSP protocol in order to achieve high cell viability and intracellular uptake of MR contrast agents. First, we determined the sub-optimal MSP parameters by evaluating the viabilities of C17.2 neural stem cells without Feridex using various MSP intensities ranging from 0.1 to 1 w/cm2, duty cycles at 20%, 50% or 100%, and exposure times from 1–15 min. The sub-optimized MSP parameters with cell viabilities greater than 90% were further optimized by evaluating both cell viability and intracellular iron uptake when Feridex was used. We then used the optimized MSP parameters to determinate the optimal concentration of Feridex for magnetic cell labeling. Subsequently, we validated the feasibility of using MRI to track the migration of neural stem cells from the transplanted sites to glioma masses in four mouse brains when the cells had been labeled with Feridex using the optimized MSP protocol. The MRI findings were confirmed by histological correlations. Invitro experiments demonstrated that the optimal MSP protocol was achieved at 20% duty cycle, 0.3 w/cm2 ultrasound intensity, 5-min exposure time and 1mg/mL Feridex. This study demonstrated that the optimized MSP cell labeling technique can achieve both high cell viability and intracellular uptake of MR contrast agents, and has the potential to be a useful cell labeling technique to facilitate future clinical translation of MRI-integrated cell therapy. Copyright © 2010 John Wiley & Sons, Ltd.

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