Manganese enhancement in non-CNS organs
Article first published online: 19 MAY 2010
Copyright © 2010 John Wiley & Sons, Ltd.
NMR in Biomedicine
Volume 23, Issue 8, pages 931–938, October 2010
How to Cite
Lee, L.-W., So, P.-W., Price, A. N., Parkinson, J. R. C., Larkman, D. J., Halliday, J., Poucher, S. M., Pugh, J. A. T., Cox, A. G., McLeod, C. W. and Bell, J. D. (2010), Manganese enhancement in non-CNS organs. NMR Biomed., 23: 931–938. doi: 10.1002/nbm.1513
- Issue published online: 19 MAY 2010
- Article first published online: 19 MAY 2010
- Manuscript Accepted: 9 JAN 2010
- Manuscript Revised: 27 NOV 2009
- Manuscript Received: 3 DEC 2008
- Medical Research Council
- non CNS organs;
Manganese-enhanced magnetic resonance imaging (MEMRI) is a novel imaging technique capable of monitoring calcium influx, in vivo. Manganese (Mn2+) ions, similar to calcium ions (Ca2+), are taken up by activated cells where their paramagnetic properties afford signal enhancement in T1-weighted MRI methodologies. In this study we have assessed Mn2+ distribution in mice using magnetization-prepared rapid gradient echo (MP-RAGE) based MRI, by measuring changes in T1–effective relaxation times (T1-eff), effective R1-relaxation rates (R1-eff) and signal intensity (SI) profiles over time. The manganese concentration in the tissue was also determined using inductively coupled plasma atomic emission spectrometry (ICP-AES). Our results show a strong positive correlation between infused dose of MnCl2 and the tissue manganese concentration. Furthermore, we demonstrate a linear relationship between R1-eff and tissue manganese concentration and tissue-specific Mn2+ distribution in murine tissues following dose-dependent Mn2+ administration. This data provides an optimized MnCl2 dose regimen for an MP-RAGE based sequence protocol for specific target organs and presents a potential 3D MRI technique for in vivo imaging of Ca2+ entry during Ca2+-dependent processes in a wide range of tissues. Copyright © 2010 John Wiley & Sons, Ltd.