Glucose metabolism via the pentose phosphate pathway, glycolysis and Krebs cycle in an orthotopic mouse model of human brain tumors (pages 1177–1186)
Isaac Marin-Valencia, Steve K. Cho, Dinesh Rakheja, Kimmo J. Hatanpaa, Payal Kapur, Tomoyuki Mashimo, Ashish Jindal, Vamsidhara Vemireddy, Levi B. Good, Jack Raisanen, Xiankai Sun, Bruce Mickey, Changho Choi, Masaya Takahashi, Osamu Togao, Juan M. Pascual, Ralph J. DeBerardinis, Elizabeth A. Maher, Craig R. Malloy and Robert M. Bachoo
Article first published online: 1 MAR 2012 | DOI: 10.1002/nbm.2787
[1,2-13C2]glucose infusion and ex vivo 13C NMR analysis of mouse orthotopic glioblastoma and renal cell carcinoma metastatic to brain demonstrate that the rate of glycolysis is significantly greater than the pentose phosphate pathway flux in these tumors. The analysis of 13C multiplets of γ-aminobutyric acid (GABA) in the renal metastasis suggests that GABA does not derive from a common glutamate precursor pool. We have shown that the renal metastasis and primary renal tumor produce GABA, which is a potential novel biomarker in this disease.