NMR in Biomedicine

Cover image for Vol. 25 Issue 9

September 2012

Volume 25, Issue 9

Pages i–ii, 1015–1112

  1. Issue Information

    1. Top of page
    2. Issue Information
    3. Research Articles
    4. Corrigendum
    1. Issue Information (pages i–ii)

      Version of Record online: 14 AUG 2012 | DOI: 10.1002/nbm.2758

  2. Research Articles

    1. Top of page
    2. Issue Information
    3. Research Articles
    4. Corrigendum
    1. Multislice fractional ventilation imaging in large animals with hyperpolarized gas MRI (pages 1015–1025)

      Kiarash Emami, Yinan Xu, Hooman Hamedani, Yi Xin, Harrilla Profka, Jennia Rajaei, Stephen Kadlecek, Masaru Ishii and Rahim R. Rizi

      Version of Record online: 31 JAN 2012 | DOI: 10.1002/nbm.2763

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      Multislice measurements of fractional ventilation with hyperpolarized (HP) gas MRI was performed for the first time in five Yorkshire pigs. This technique utilizes a series of back-to-back HP gas breaths with images acquired during short end-inspiratory breath-holds. Flip angle maps were estimated by acquiring a series of back-to-back images with no interscan time delay during a separate breath-hold. Results demonstrate the feasibility of robust measurements of fractional ventilation in large species, and provide a platform for translation to humans

    2. Apparent diffusion coefficient of hyperpolarized 3He with minimal influence of the residual gas in small animals (pages 1026–1032)

      L. Carrero-González, T. Kaulisch, J. Ruiz-Cabello, J. M. Pérez-Sánchez, G. Peces-Barba, D. Stiller and I. Rodríguez

      Version of Record online: 25 JAN 2012 | DOI: 10.1002/nbm.2765

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      The application of a positive end-expiratory pressure increases the residual gas volume in the lung. This increase affects the ratio between the incoming/residual gas volumes and therefore changes the ADC if both gases do not have the same intradiffusion coefficient. A ventilation method (ventilation with an oxygen–helium mixture, VOHeM) using two different gas mixtures (one for ventilation and one for imaging) is proposed to minimize the residual gas effect on ADC measurements. ADC is acquired in a single breath-hold, reducing the amount of hyperpolarized gas.

    3. Glycerophosphodiester phosphodiesterase domain containing 5 (GDPD5) expression correlates with malignant choline phospholipid metabolite profiles in human breast cancer (pages 1033–1042)

      Maria D. Cao, Mailin Döpkens, Balaji Krishnamachary, Farhad Vesuna, Mayur M. Gadiya, Per E. Lønning, Zaver M. Bhujwalla, Ingrid S. Gribbestad and Kristine Glunde

      Version of Record online: 26 JAN 2012 | DOI: 10.1002/nbm.2766

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      The gene(s) encoding for the glycerophosphocholine phosphodiesterase (GPC-PDE) responsible for glycerophosphocholine (GPC) degradation in breast cancers have not yet been identified. Here, we showed for the first time that the GPC-PDE encoded by glycerophosphodiester phosphodiesterase domain containing 5 (GDPD5) was significantly overexpressed, together with choline kinase alpha (CHKA) and phosphatidylcholine-specific phospholipase D1 (PLD1), in estrogen receptor-negative (ER) breast tumors with a malignant choline metabolite profile of low GPC and high phosphocholine (PC). Our study identified GDPD5 as a GPC-PDE that probably participates in the regulation of choline phospholipid metabolism in breast cancer in cooperation with CHKA and PLD1.

    4. A novel method for simultaneous 3D B1 and T1 mapping: the method of slopes (MoS) (pages 1043–1055)

      Sofia Chavez and Greg J. Stanisz

      Version of Record online: 26 FEB 2012 | DOI: 10.1002/nbm.2769

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      A new method is presented, yielding simultaneous, consistent, 3D B1 and T1 maps at 3T in a reasonable scan time. The SPGR signal is sampled at flip angles where the signal varies linearly as a function of flip angle. Main improvements on the variable flip angle T1 mapping method: (i) consistent B1 correction (ii) T1- independent sampling scheme (iii) elimination of noise bias and singularity associated with using the linear form of the SPGR signal equation.

    5. Assessment of the dentin-pulp complex response to caries by ADC mapping (pages 1056–1062)

      Jernej Vidmar, Ksenija Cankar, Lidija Nemeth and Igor Serša

      Version of Record online: 16 JAN 2012 | DOI: 10.1002/nbm.2770

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      The prognostic potential of ADC mapping in the assessment of dentin-pulp complex response to caries was studied. ADC maps of teeth enabled discrimination between intact (ADC > 1.0·10-9 m2/s) and affected (ADC < 1.0·10-9 m2/s) regions of dental pulp. The analyses between the demineralization depth, caries scoring (ICDAS) and average ADC showed that the demineralization increase of one millimeter results in an ICDAS score increase of 1.2 and in an average ADC decrease of 0.07·10-9 m2/s.

    6. Mean cerebral blood flow measurements using phase contrast MRI in the first year of life (pages 1063–1072)

      Marta Varela, Alan M Groves, Tomoki Arichi and Joseph V Hajnal

      Version of Record online: 31 JAN 2012 | DOI: 10.1002/nbm.2771

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      We present a method capable of measuring mean cerebral blood flow (CBF) non-invasively in neonates and small infants. Mean CBF is computed by dividing flow to the brain, measured using phase-contrast angiography, by brain volume, estimated from the segmentation of an anatomical MR image. Image acquisition and analysis was optimized for small infants. This method was used to measure mean CBF in 21 infants rapidly and non-invasively, providing values in good agreement with literature when available.

    7. Increased ventricular lactate in chronic fatigue syndrome. III. Relationships to cortical glutathione and clinical symptoms implicate oxidative stress in disorder pathophysiology (pages 1073–1087)

      Dikoma C. Shungu, Nora Weiduschat, James W. Murrough, Xiangling Mao, Sarah Pillemer, Jonathan P. Dyke, Marvin S. Medow, Benjamin H. Natelson, Julian M. Stewart and Sanjay J. Mathew

      Version of Record online: 27 JAN 2012 | DOI: 10.1002/nbm.2772

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      Pathophysiological model of chronic fatigue syndrome (CFS) which attempts to explain the consistent observation of cross-sectional elevations of ventricular lactate in the disorder. Highlighted are the experimentally measurable items, with the red arrows showing the model-predicted outcomes. This series of neuroimaging studies aimed to validate this model by measuring each key item and comparing the results with the model-predicted outcomes.

    8. High-resolution NMR spectroscopy in inhomogeneous fields via Hadamard-encoded intermolecular double-quantum coherences (pages 1088–1094)

      Yushan Chen, Shuhui Cai, Congbo Cai, Xiaohong Cui and Zhong Chen

      Version of Record online: 31 JAN 2012 | DOI: 10.1002/nbm.2773

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      A new pulse sequence based on intermolecular double-quantum coherences was proposed to obtain high-resolution one-dimensional NMR spectra in inhomogeneous magnetic fields via Hadamard encoding. Solution and pig brain tissue samples were tested. Experimental results demonstrate that this sequence can retain useful spectral information, even when the field inhomogeneity is sufficiently severe to erase almost all spectral information with conventional 1D single-quantum coherence techniques, and good solvent suppression can be achieved.

    9. A large-scale 19F MRI-based cell migration assay to optimize cell therapy (pages 1095–1103)

      F. Bonetto, M. Srinivas, B. Weigelin, L. J. Cruz, A. Heerschap, P. Friedl, C. G. Figdor and I. J. M. de Vries

      Version of Record online: 7 FEB 2012 | DOI: 10.1002/nbm.2774

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      A novel 19 F MR-based, large-scale quantitative assay to measure cell migration in three-dimensional opaque samples is introduced. Migration rates in the present assay were assessed for different cell densities of 19F-labeled human dendritic cells using two different methods. No migration was found for high cell densities, and up to 3% was found for low cell densities. Consequently, we show that cell density has a decisive impact on migration in cell bolus injections.

    10. Early prediction of response to Vorinostat in an orthotopic rat glioma model (pages 1104–1111)

      Li Wei, Samuel Hong, Younghyoun Yoon, Scott N. Hwang, Jaekeun C. Park, Zhaobin Zhang, Jeffrey J. Olson, Xiaoping P. Hu and Hyunsuk Shim

      Version of Record online: 2 FEB 2012 | DOI: 10.1002/nbm.2776

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      During carcinogenesis, tumor suppressor genes can be silenced by aberrant histone deacetylation caused by histone deacetylase (HDAC). A major shortcoming of the use of HDAC inhibitors in the treatment of patients with brain tumors is the lack of reliable prognostic biomarkers. The current work describes a preclinical study for the development of MRS-based and histopathologic biomarkers, which correlate with the normalization of brain metabolism and function, and thus predict the therapeutic response to Vorinostat.

  3. Corrigendum

    1. Top of page
    2. Issue Information
    3. Research Articles
    4. Corrigendum
    1. Corrigendum (page 1112)

      Weiliang Du, Xiaobing Fan, Sean Foxley, Marta Zamora, Jonathan N. River, Rita M. Culp and Gregory S. Karczmar

      Version of Record online: 25 JUL 2012 | DOI: 10.1002/nbm.2838