Establishment and characterization of multipotent neural cell lines using retrovirus vector-mediated oncogene transfer

Authors

  • Elizabeth F. Ryder,

    1. Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
    Search for more papers by this author
  • Evan Y. Snyder,

    1. Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
    2. Department of Neurology, and Pediatrics, Harvard Medical School, Boston, Massachusetts 02115
    Search for more papers by this author
  • Constance L. Cepko

    Corresponding author
    1. Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
    • Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
    Search for more papers by this author

Abstract

Neural cell lines were produced by retroviral vector-mediated transduction of the avian myc oncogene. Target cells were mitotic progenitor cells of postnatal mouse olfactory bulb and cerebellum, and postnatal rat cerebral cortex. Infection of the first two areas, where neurogenesis and gliogenesis occur postnatally, produced multipotent clonal lines that exhibited phenotypes of both neuronal and glial cells, and one line with a stable neuronal phenotype. Infection of cerebral cortex, where gliogenesis, but not neurogenesis, occurs postnatally, generated mortal clones that exhibited cells of glial phenotype. These lines should prove valuable for both in vitro and in vivo studies aimed at understanding the control of cell fate and differentiation of neural progenitors.

Ancillary