Diagnostic morphometric applicability of confocal laser scanning microscopy in Osteoarchaeology
Version of Record online: 29 APR 2009
Copyright © 2009 John Wiley & Sons, Ltd.
International Journal of Osteoarchaeology
Volume 20, Issue 6, pages 708–718, November/December 2010
How to Cite
Papageorgopoulou, C., Kuhn, G., Ziegler, U. and Rühli, F. J. (2010), Diagnostic morphometric applicability of confocal laser scanning microscopy in Osteoarchaeology. Int. J. Osteoarchaeol., 20: 708–718. doi: 10.1002/oa.1078
- Issue online: 29 APR 2009
- Version of Record online: 29 APR 2009
- Manuscript Accepted: 3 FEB 2009
- Manuscript Revised: 22 JAN 2009
- Manuscript Received: 23 JUL 2008
- Bone microdamage;
- 3D imaging
The confocal laser scanning microscope (CLSM) has become an essential tool for a wide range of biological and clinical studies and imaging applications. The major imaging modes of the CLSM include single and serial optical sections from thick specimens (100 µm), multiple wavelength images and three-dimensional reconstruction. Such images provide measurement of length, surface or volume of objects. Surprisingly, this advanced microscopic method has been very rarely used in anthropological research, although numerous clinical and basic science research projects showed its value to study, bone growth, bone micro-architecture and 3D bone morphometry. We present the basic principles and advantages of CLSM and outline practical aspects of specimen preparation, image collection and digital image processing specifically appropriate for historical bone and hair material. Copyright © 2009 John Wiley & Sons, Ltd.