Manual preparation of ground sections for the microscopy of natural bone tissue: update and modification of Frost's ‘rapid manual method’
Article first published online: 21 SEP 2001
Copyright © 2001 John Wiley & Sons, Ltd.
International Journal of Osteoarchaeology
Volume 11, Issue 5, pages 366–374, September/October 2001
How to Cite
Maat, G. J.R., Van Den Bos, R. P.M. and Aarents, M. (2001), Manual preparation of ground sections for the microscopy of natural bone tissue: update and modification of Frost's ‘rapid manual method’. Int. J. Osteoarchaeol., 11: 366–374. doi: 10.1002/oa.578
- Issue published online: 21 SEP 2001
- Article first published online: 21 SEP 2001
- Manuscript Revised: 12 SEP 2000
- Manuscript Accepted: 12 SEP 2000
- Manuscript Received: 9 AUG 2000
- ground section;
- manual technique
Light microscopy should be a routine complementary diagnostic tool for osteoarchaeologists, palaeopathologists and forensic anthropologists. However, this well-established and long existing technique is not widely utilized. It is a popular belief that the preparation of the required sections of bone tissue demands a lot of time, expensive instruments, and a knowledge of plastics. To show that this is not entirely the case, a long existing technique for the manual preparation of ground sections was brushed up and tested. Its application is simple, extremely cheap, rapid and reliable and the final product is a beautiful and intact ca. 10–15 micron or less thin section. A few modifications are proposed which further improve the feasibility of the technique, and which also make it very suitable for less well-preserved, inhumed, and even cremated osteoarchaeological/forensic remains. As in the original method, the modifications need only a few extra, but still very basic and cheap products. A detailed step by step description of the procedure is presented. The advantages are then discussed. Copyright © 2001 John Wiley & Sons, Ltd.