A cross-sectional study of osteocalcin and body fat measures among obese adolescents

Authors


  • Disclosure: The authors have nothing to disclose.

  • Funding agencies: Dr. Lenders received support from the Joel and Barbara Alpert Endowment for the Children of the City, the NIH grant MO1-RR00533 (Boston University School of Medicine, Michael F. Holick). We are very thankful for the contribution of Howard Bauchner in the review of the manuscript with support from his 5 K24 HD042489. The GPRN is funded by the Elizabeth Glaser Pediatric Research Foundation (EGPRF), a program of the Elizabeth Glaser Pediatric AIDS Foundation. The study was funded by the EGPRF and the NIH/NCRR (Stanford University: grant number MO1-RR00070; Baylor College of Medicine: grant number MO1-RR00188; University of California, San Francisco: UCSF-CTSI grant number UL-RR024131-01; University of California, Los Angeles: grant number MO1-RR00865; Harvard Medical School: Children's Hospital Boston grant number MO1-RR02172).

Abstract

Osteocalcin (OCN), a marker of osteoblast activity, has been implicated in the regulation of energy metabolism by the skeleton and thus may affect body fat measures.

Objective:

To examine the relationships of OCN to body fat measures and whether they vary according to markers of energy and vitamin D metabolism.

Design and Methods:

Data were obtained from 58 obese adolescents aged 13-17.9 years (38 females, 8 black or African-American). Total fat mass (FM) [dual X-ray absorptiometry (DXA)] and visceral adipose tissue (VAT) [computerized axial tomography (CT)] were calculated. Blood tests included leptin, OCN, 25-hydroxyvitamin D [25(OH)D], parathyroid hormone (PTH), thyroid function tests, and triglycerides. Markers of glucose metabolism were obtained from fasting and OGTT samples.

Results and Conclusions:

Adolescents with 25(OH)D <20 ng mL−1 were considered deficient (n = 17/58); none had high PTH (PTH ≥ 65 pg mL−1). OCN was associated with lower VAT (−84.27 ± 33.89 mm2) and BMI (−0.10 ± 0.05 kg m−2), not FM (P = 0.597) in a core model including age, sex, race, geographic latitude, summer, height z-score, and tanner stage. Adding 25(OH)D deficiency and PTH attenuated the inverse association of OCN to VAT. There was a significant interaction of OCN and 25(OH)D deficiency on FM (0.37 ± 0.18 kg, P = 0.041) and BMI (0.28 ± 0.10 kg m−2, P = 0.007) in this adjusted model, which was further explained by leptin. Adding A1C to the core model modified the relationship of OCN to VAT (−93.08 ± 35.05 mm2, P = 0.011), which was further explained by HOMA-IR. In summary, these findings provide initial evidence for a relationship between OCN and body fat measures that is dependent on energy metabolism and vitamin D status among obese adolescents.

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