Disclosure: The authors declared no conflict of interest.
Associations among SPARC mRNA expression in adipose tissue, serum SPARC concentration and metabolic parameters in Korean women
Article first published online: 13 MAY 2013
Copyright © 2012 The Obesity Society
Volume 21, Issue 11, pages 2296–2302, November 2013
How to Cite
Lee, S.H., Lee, J.A., Park, H.S., Song, Y.S., Jang, Y.J., Kim, J.H., Lee, Y.J. and Heo, Y. (2013), Associations among SPARC mRNA expression in adipose tissue, serum SPARC concentration and metabolic parameters in Korean women. Obesity, 21: 2296–2302. doi: 10.1002/oby.20183
Funding agencies: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology (2010-0021599) and a grant from the Asan Institute for Life Sciences (2012-106) Seoul, Korea.
- Issue published online: 1 NOV 2013
- Article first published online: 13 MAY 2013
- Accepted manuscript online: 29 NOV 2012 04:13PM EST
- Manuscript Accepted: 12 NOV 2012
- Manuscript Received: 15 DEC 2011
Objective: Secreted protein acidic and rich in cysteine (SPARC) is expressed in most tissues and is also secreted by adipocytes. The associations of SPARC mRNA expression in visceral adipose tissue (VAT), subcutaneous abdominal adipose tissue (SAT), serum SPARC concentration, and metabolic parameters in Korean women are investigated.
Design and Methods: This is a cross-sectional study. Fifty-eight women were recruited, of whom 15 women who underwent bariatric surgery for morbid obesity (BMI mean ± SD: 40.2±5.7 kg/m2), 16 who underwent metabolic surgery for type 2 diabetes (BMI: 28.9±4.5 kg/m2), and, as a control group, 27 who underwent gynecological surgery (BMI: 22.7±2.4 kg/m2). Anthropometric variables, metabolic parameters, SPARC mRNA expression in adipose tissue, and serum SPARC concentration were measured.
Results: In all subjects, SPARC mRNA expression was significantly higher in SAT than in VAT. Serum SPARC concentrations (mean ± SE) in morbidly obese subjects, subjects with type 2 diabetes, and normal weight subjects were 267.3±40.2 ng/mL, 130.4±33.0 ng/mL, and 53.1±2.8 ng/mL, respectively. SPARC mRNA in SAT was significantly correlated with BMI, whereas SPARC mRNA in VAT was significantly correlated with BMI and VAT area. Serum SPARC concentration was significantly correlated with BMI, waist circumference, total adipose tissue area, and SAT area. After BMI adjustment, serum SPARC concentration was significantly correlated with fasting insulin concentration and HOMA-IR score. Multivariate regression analysis showed that BMI and HOMA-IR were independently associated with serum SPARC concentration.
Conclusions: Serum SPARC concentration is significantly correlated with obesity indices and might be influenced by insulin resistance. These findings suggest that SPARC may contribute to the metabolic dysregulation associated with obesity in humans.