Author contributions: Wei-Dong Li: study design, oversee and conduct experiments, analyze data, write the manuscript; Hongxiao Jiao: analyze data, comment on the manuscript; Kai Wang: analyze data, comment on the manuscript; Clarence Zhang: analyze data; Joseph T Glessner: oversee and conduct experiments; Struan F.A. Grant: oversee and conduct experiments; Hongyu Zhao: oversee and conduct data analyses; Hakon Hakonarson: oversee and conduct experiment, study design; R. Arlen Price: study design, oversee experiments and analyze data, write and comment on the manuscript.
A genome wide association study of plasma uric acid levels in obese cases and never-overweight controls
Version of Record online: 24 MAY 2013
Copyright © 2013 The Obesity Society
Volume 21, Issue 9, pages E490–E494, September 2013
How to Cite
Li, W.-D., Jiao, H., Wang, K., Zhang, C. K., Glessner, J. T., Grant, S. F.A., Zhao, H., Hakonarson, H. and Arlen Price, R. (2013), A genome wide association study of plasma uric acid levels in obese cases and never-overweight controls. Obesity, 21: E490–E494. doi: 10.1002/oby.20303
Disclosure: The authors declared no conflict of interest.
- Issue online: 23 SEP 2013
- Version of Record online: 24 MAY 2013
- Accepted manuscript online: 2 JAN 2013 07:15PM EST
- Manuscript Accepted: 24 NOV 2012
- Manuscript Received: 7 SEP 2011
To identify plasma uric acid-related genes in extremely obese and normal weight individuals using genome-wide association studies (GWASs).
Design and Methods
Using genotypes from a GWAS focusing on obesity and thinness, quantitative trait association analyses (PLINK) for plasma uric acid levels in 1,060 extremely obese individuals (BMI > 35 kg/m2) and normal-weight controls (BMI < 25kg/m2) were performed. In 961 samples with uric acid data, 924 were females.
Significant associations were found in SLC2A9 gene SNPs and plasma uric acid levels (rs6449213, P = 3.15 × 10–12). DIP2C gene SNP rs877282 also reached genome-wide significance (P = 4.56 × 10–8). Weaker associations (P < 1× 10–5) were found in F5, PXDNL, FRAS1, LCORL, and MICAL2 genes. Besides SLC2A9, three previously identified uric acid-related genes ABCG2 (rs2622605, P= 0.0026), SLC17A1 (rs3799344, P = 0.0017), and RREB1 (rs1615495, P = 0.00055) received marginal support in our study.
Two genes/chromosome regions reached genome-wide association significance (P < 1 × 10–7, 550K SNPs) in our GWAS: SLC2A9, the chromosome 2 60.1 Mb region (rs6723995), and the DIP2C gene region. Five other genes (F5, PXDNL, FRAS1, LCORL, and MICAL2) yielded P < 1 × 10–5. Four previous reported associations were replicated in our study, including SLC2A9, ABCG2, RREB, and SLC17A1.