Disclosure: The authors have no competing interests.
Diet composition modulates expression of sirtuins and Renin-Angiotensin system components in adipose tissue
Article first published online: 24 MAY 2013
Copyright © 2013 The Obesity Society
Volume 21, Issue 9, pages 1830–1835, September 2013
How to Cite
de Pinho, L., Andrade, J. M. O., Paraíso, A., Filho, A. B. M., Feltenberger, J. D., Guimarães, A. L. S., de Paula, A. Mauricio. B., Caldeira, A. P., de Carvalho Botelho, A. C., Campagnole-Santos, M. J. and Sousa Santos, S. H. (2013), Diet composition modulates expression of sirtuins and Renin-Angiotensin system components in adipose tissue. Obesity, 21: 1830–1835. doi: 10.1002/oby.20305
- Issue published online: 23 SEP 2013
- Article first published online: 24 MAY 2013
- Accepted manuscript online: 14 FEB 2013 07:12AM EST
- Manuscript Accepted: 30 NOV 2012
- Manuscript Received: 1 AUG 2012
The aim of this study was to evaluate the expression of RAS components and SIRTs enzymes in the adipose tissue of mice fed diets with different macronutrient composition.
Design and Methods
The body weight, food intake, and energy intake (kcal) were evaluated. Blood parameters (insulin sensitivity, glucose tolerance, total cholesterol, HDL-C triglyceride, and glucose levels) were also assessed. Real-time PCR was performed in epididymal adipose tissue samples to analyze the expression of renin, angiotensinogen (AGT), angiotensin-converting enzyme 1 and 2 (ACE and ACE2), and SIRTs 1-7. Male FVB/N mice were divided into 5 groups (N = 10 each) that were fed with experimental diets for 60 days. Test diets were divided into standard (ST), AIN-93M, high glucose (HG), high protein (HP) and high lipid (HL).
The main results showed that HL diet treatment induced reduction in HDL-C and triglyceride plasma levels; increased ACE (Ang II marker) expression and decreased ACE2 (Ang-[1-7] catalyzer) expression in adipose tissue; and also increased SIRT4 expression.
Diets with high lipid content induced a degenerative state associated with deregulation of adipose tissue enzymes expression.