Disclosure: The authors declared no conflict of interest.
Structural and functional microvascular alterations in a rat model of metabolic syndrome induced by a high-fat diet
Article first published online: 29 MAY 2013
Copyright © 2013 The Obesity Society
Volume 21, Issue 10, pages 2046–2054, October 2013
How to Cite
Nascimento, A. R., Machado, M., de Jesus, N., Gomes, F., Lessa, M. A., Bonomo, I. T. and Tibiriçá, E. (2013), Structural and functional microvascular alterations in a rat model of metabolic syndrome induced by a high-fat diet. Obesity, 21: 2046–2054. doi: 10.1002/oby.20358
- Issue published online: 5 OCT 2013
- Article first published online: 29 MAY 2013
- Accepted manuscript online: 20 MAR 2013 01:54AM EST
- Manuscript Accepted: 16 DEC 2012
- Manuscript Received: 27 FEB 2012
To investigate microvascular alterations in an experimental model of metabolic syndrome induced by a high-fat diet (HFD) associated with salt supplementation (0.5% NaCl).
Design and Methods
Wistar Kyoto rats were fed standard chow (control group, CONT) or HFD for 20 weeks. The functional capillary density (FCD) was assessed using intravital fluorescence videomicroscopy.
The HFD group presented a higher systolic blood pressure, plasma glucose and insulin levels, total and LDL-cholesterol levels, triglycerides, and visceral and epididymal fat when compared with the CONT group. When compared with the CONT group, the HFD group showed a lower FCD in the skeletal muscle (P < 0.05) but not in the skin (P > 0.05). The HFD group also had a lower capillary-to-fiber ratio in the skeletal muscle (P < 0.01). The capillary volume density-to-fiber volume density ratio in the left ventricle of the HFD was also reduced (P < 0.01). Finally, rats fed with HFD showed ventricular hypertrophy and increased cardiomyocyte diameter (P < 0.01).
The long-term administration of a HFD associated with salt supplementation to rats generates an experimental model of metabolic syndrome characterized by central body fat deposition, insulin resistance, glucose intolerance, hypertriglyceridemia, hypercholesterolemia, arterial hypertension, cardiac remodeling, and rarefaction of the microcirculation in the heart and skeletal muscle.