Increased β-oxidation with improved glucose uptake capacity in adipose tissue from obese after weight loss and maintenance§

Authors

  • Freek G. Bouwman,

    1. Department of Human Biology, NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Centre, Maastricht, The Netherlands
    Search for more papers by this author
  • Ping Wang,

    1. Department of Human Biology, NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Centre, Maastricht, The Netherlands
    Search for more papers by this author
  • Marleen van Baak,

    1. Department of Human Biology, NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Centre, Maastricht, The Netherlands
    Search for more papers by this author
  • Wim H.M. Saris,

    1. Department of Human Biology, NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Centre, Maastricht, The Netherlands
    Search for more papers by this author
  • Edwin C.M. Mariman

    Corresponding author
    1. Department of Human Biology, NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Centre, Maastricht, The Netherlands
    • Department of Human Biology, NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Centre, Maastricht, The Netherlands. E-mail: e.mariman@maastrichtuniversity.nl

    Search for more papers by this author

  • Funding agencies: This research was supported by the Maastricht Proteomics Center of Maastricht University.

  • §

    Disclosure: The authors declared no conflict of interest.

  • Author contributions: FB, PW, MB, WS, and EM conception and design of research; FB performed experiments; FB analyzed data; FB, PW, and EM interpreted results of experiments; FB prepared figures; all authors were involved in writing the article and had final approval of the submitted and published versions.

Abstract

Objective

We investigated protein markers for pathways of the fatty acids (FAs) and glucose metabolism in human adipose tissue after a weight loss program by calorie restriction.

Methods

Overweight/obese subjects underwent an intervention of 5 weeks of a very low-calorie diet followed by a 3-week weight maintenance diet. Abdominal subcutaneous adipose tissue biopsies were sampled before and after the intervention. Seventeen target proteins as markers of metabolic pathways for the uptake and handling of FAs and glucose were quantified by Western blotting and 11 were retrieved from previous proteomics work. Correlation coefficients were calculated among changes of these proteins.

Results

Short-chain 3-hydroxyacyl-CoA dehydrogenase, catalase, fatty acid translocase, fatty acid transporter protein 3, adipose triglyceride lipase, fatty acid-binding protein 4, aldolase-C, tubulin-β-5, and annexin A2 changed significantly, and lipoprotein lipase, perilipin 1, and hormone-sensitive lipase tended to change. On an average, increased glucose transporter type 4 translocation was observed, supported by a consistent increase of tyr-24 phosphorylated annexin A2.

Conclusions

Our findings suggest that after weight loss by calorie restriction and a short period of maintenance, adipose tissue has an increased capacity for glucose uptake, and lipid mobilization and oxidation. Such metabolic profile may relate to the health benefit of weight loss.

Ancillary