Disclosure: The authors declared no conflict of interest.
Overweight and obese adult humans have a defective cellular immune response to pandemic H1N1 Influenza a virus
Article first published online: 30 MAY 2013
Copyright © 2013 The Obesity Society
Volume 21, Issue 11, pages 2377–2386, November 2013
How to Cite
Paich, H. A., Sheridan, P. A., Handy, J., Karlsson, E. A., Schultz-Cherry, S., Hudgens, M. G., Noah, T. L., Weir, S. S. and Beck, M. A. (2013), Overweight and obese adult humans have a defective cellular immune response to pandemic H1N1 Influenza a virus. Obesity, 21: 2377–2386. doi: 10.1002/oby.20383
Relevant conflicts of interest/financial disclosures: Nothing to report. Full financial disclosures and author notes may be found in the online version of this article.
Funding agencies: This work was supported by the National Institutes of Health Grants NIH ROI AI078090 (to M.A.B.) and P30DK056350 (to M.A.B.), by NIH NIAID contract number HHSN266200700005C (to S.S.C.) and by the American Lebanese Syrian Associated Charities (to S.S.C.). This research was supported by the National Institutes of Health Grants NIH ROI AI078090 and P30DK056350, by NIH NIAID grant number HHSN266200700005C, and by the American Lebanese Syrian Associated Charities.
- Issue published online: 1 NOV 2013
- Article first published online: 30 MAY 2013
- Accepted manuscript online: 20 MAR 2013 02:10AM EST
- Manuscript Accepted: 28 DEC 2012
- Manuscript Received: 12 NOV 2012
- The National Institutes of Health Grants NIH ROI. Grant Numbers: AI078090, P30DK056350
- NIH NIAID. Grant Number: HHSN266200700005C
- The American Lebanese Syrian Associated Charities
Obese adults have a greater risk of morbidity and mortality from infection with pandemic H1N1 influenza A virus (pH1N1). The objective of the present study was to elucidate the specific mechanisms by which obesity and overweight impact the cellular immune response to pH1N1.
Design and Methods
Peripheral blood mononuclear cells from healthy weight, overweight, and obese individuals were stimulated ex vivo with live pH1N1 and then markers of activation and function were measured using flow cytometry and cytokine secretion was measured using cytometric bead array assays.
CD4+ and CD8+ T cells from overweight and obese individuals expressed lower levels of CD69, CD28, CD40 ligand, and interleukin-12 receptor, as well as, produced lower levels of interferon-γ and granzyme B, compared with healthy weight individuals, suggesting deficiencies in activation and function are indicated. Dendritic cells from the three groups expressed similar levels of major histocompatibility complex-II, CD40, CD80, and CD86, as well as, produced similar levels of interleukin-12.
The defects in CD4+ and CD8+ T cells may contribute to the increased morbidity and mortality from pH1N1 in obese individuals. These data also provide evidence that both overweight and obesity cause impairments in immune function.