Funding agencies: Clinical trial reg. no. NCT00697580, clinicaltrials.gov. This work was supported by the following: The Robert C. and Veronica Atkins Foundation Grant, and USC Graduate Provost Fellowship. The content is solely the responsibility of the authors and does not necessarily represent the official views of the sponsoring agencies.
Brief Cutting Edge Report
Adipose tissue 11βHSD1 gene expression, βcell function and ectopic fat in obese African Americans versus Hispanics
Version of Record online: 10 SEP 2013
Copyright © 2013 The Obesity Society
Volume 22, Issue 1, pages 14–18, January 2014
How to Cite
Gyllenhammer, L. E., Alderete, T. L., Mahurka, S., Allayee, H. and Goran, M. I. (2014), Adipose tissue 11βHSD1 gene expression, βcell function and ectopic fat in obese African Americans versus Hispanics. Obesity, 22: 14–18. doi: 10.1002/oby.20571
Disclosure: The authors have no competing interests to report.
Author contributions: L.E.G. designed the study, analyzed data, wrote the manuscript, and reviewed and edited the manuscript. S.M., H.A., M.I.G. reviewed and edited the manuscript. T.L.A. contributed to data and sample collection and reviewed and edited the manuscript.
- Issue online: 11 JAN 2014
- Version of Record online: 10 SEP 2013
- Accepted manuscript online: 9 JUL 2013 01:59AM EST
- Manuscript Accepted: 21 JUN 2013
- Manuscript Received: 31 JAN 2013
This study examined the contribution of subcutaneous adipose tissue (SAT) 11βHSD1 to obese African Americans' (AA) elevated metabolic risk, despite a protective obesity phenotype of reduced visceral adipose tissue (VAT) and hepatic fat fraction (HFF) relative to obese Hispanics with similar metabolic risk.
Design and Methods
Obese AA and Hispanic adults (N = 36(16AA); BMI 35.2 ± 0.6 kg/m2, 18-25y) participated, with VAT, SAT, and HFF measured by MRI, SAT gene expression measured by HT-12 microarray and insulin sensitivity (SI), disposition index (DI) by IVGTT. Multiple linear regression examined relationships/interactions of ethnicity and 11βHSD1 expression on outcomes (covariates: age, sex, total fat mass), with standardized β (stβ) reported.
SAT 11βHSD1 expression significantly associated with insulin parameters and this varied by ethnicity (Pinteraction<0.1). In AA, 11βHSD1 negatively associated with SI (stβ = -0.58, P = 0.03), DI (stβ = −0.62, P = 0.03) and positively associated with fasting insulin (stβ = 0.54, P = 0.04), with no significant relationship in Hispanics. SAT 11βHSD1 associated with HFF in the combined sample (stβ = 0.42, P = 0.008), with no difference between ethnicites (Pinteraction>0.1). After controlling for HFF, 11βHSD1 associations with metabolic risk in AA became nonsignificant.
These results suggested that in AA and not Hispanics, SAT 11βHSD1 is associated with SI and DI, and may be mediated by HFF.