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Keywords:

  • affinity labels;
  • artificial metalloproteins;
  • asymmetric catalysis;
  • cysteine proteases;
  • hydrogenation
Thumbnail image of graphical abstract

How to train a protein: Metal-conjugated affinity labels were used to selectively position catalytically active metal centers in the binding pocket of proteases. The resulting artificial metalloenzymes achieve up to 82 % e.r. in the hydrogenation of ketones. The modular setup enables a rapid generation of artificial metalloenzyme libraries, which can be adapted to a broad range of catalytic conditions.