Metal-Conjugated Affinity Labels: A New Concept to Create Enantioselective Artificial Metalloenzymes
Article first published online: 20 FEB 2013
© 2013. The Authors. ChemistryOpen published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Volume 2, Issue 2, pages 50–54, April 2013
How to Cite
Reiner, T., Jantke, D., Marziale, A. N., Raba, A. and Eppinger, J. (2013), Metal-Conjugated Affinity Labels: A New Concept to Create Enantioselective Artificial Metalloenzymes. ChemistryOpen, 2: 50–54. doi: 10.1002/open.201200044
- Issue published online: 23 APR 2013
- Article first published online: 20 FEB 2013
- Manuscript Received: 15 NOV 2012
- Funded Access
- King Abdullah University of Science and Technology Global Collaborative Research (KAUST-GCR, Saudi Arabia). Grant Number: FIC/2010/07
- affinity labels;
- artificial metalloproteins;
- asymmetric catalysis;
- cysteine proteases;
How to train a protein: Metal-conjugated affinity labels were used to selectively position catalytically active metal centers in the binding pocket of proteases. The resulting artificial metalloenzymes achieve up to 82 % e.r. in the hydrogenation of ketones. The modular setup enables a rapid generation of artificial metalloenzyme libraries, which can be adapted to a broad range of catalytic conditions.