• Open Access

Rapid Microfluidic Immunoassays of Cancer Biomarker Proteins Using Disposable Inkjet-Printed Gold Nanoparticle Arrays

Authors

  • Colleen E. Krause,

    1. Department of Chemistry, University of Connecticut, Storrs, CT 0626 (USA)
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  • Brunah A. Otieno,

    1. Department of Chemistry, University of Connecticut, Storrs, CT 0626 (USA)
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  • Dr. Alina Latus,

    1. Department of Chemistry, University of Connecticut, Storrs, CT 0626 (USA)
    2. Institute of Physical Chemistry, “I. Murgulescu” Romanian Academy Splaiul, Independentei 202, Bucarest 060021 (Romania)
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  • Dr. Ronaldo C. Faria,

    1. Departamento de Quimic, Universidade Federal de Sao Carlos, Sao Paulo (Brazil)
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  • Dr. Vyomesh Patel,

    1. Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD (USA)
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  • Dr. J. Silvio Gutkind,

    1. Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD (USA)
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  • Dr. James F. Rusling

    Corresponding author
    1. Department of Chemistry, University of Connecticut, Storrs, CT 0626 (USA)
    2. Department of Cell Biology, University of Connecticut, Health Center, Farmington, CT 0623 (USA)
    3. Department of Chemistry, National University of Ireland at Galway, Galway (Ireland)
    • Department of Chemistry, University of Connecticut, Storrs, CT 0626 (USA)

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Abstract

original image

Off-line amplification: A low-cost inkjet-printed gold nanoparticle immunoarray was integrated into a microfluidic device for multiple protein determinations. The use of heavily labeled magnetic beads allows optimization either for ultrasensitive detection of biomarker proteins (low fg mL−1 levels) or for rapid 8 min assays within clinically relevant detection ranges.

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