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Special Issue: Research Article
Covalent VEGF protein immobilization on resorbable polymeric surfaces
Article first published online: 15 JUL 2010
DOI: 10.1002/pat.1772
Copyright © 2010 John Wiley & Sons, Ltd.
Additional Information
How to Cite
Edlund, U., Sauter, T. and Albertsson, A.-C. (2011), Covalent VEGF protein immobilization on resorbable polymeric surfaces. Polym. Adv. Technol., 22: 2368–2373. doi: 10.1002/pat.1772
Publication History
- Issue published online: 23 NOV 2011
- Article first published online: 15 JUL 2010
- Manuscript Accepted: 13 MAY 2010
- Manuscript Received: 23 DEC 2009
- Abstract
- Article
- References
- Cited By
Keywords:
- polylactide;
- poly(ε-caprolactone);
- vapor phase grafting;
- VEGF;
- surface
Abstract
Vascular endothelial growth factor type protein (VEGF), a potent angiogenic effector molecule, was successfully covalently immobilized onto the surfaces of the resorbable polymers poly(L-lactic acid) (PLLA) and poly(ε-caprolactone) (PCL) through a three-step strategy. The surfaces were first covalently grafted with poly(acrylic acid) using non-destructive and solvent free vapor-phase grafting. A diamine spacer was coupled to the carboxylic acid pendant groups on the graft chains using EDC/NHS chemistry and VEGF was finally covalently attached to the amine linkers. The chemistry and topography of the modified substrates were quantitatively and qualitatively verified with XPS, ATR-FTIR, UV–VIS, SEM, and ELISA. Copyright © 2010 John Wiley & Sons, Ltd.