Altered expression of the septin gene, SEPT9, in ovarian neoplasia

Authors

  • James F Burrows,

    1. Department of Oncology, Cancer Research Centre, Queen's University Belfast, Belfast City Hospital, Belfast BT9 7AB, N Ireland, UK
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    • These authors have contributed equally to this paper.

  • Severine Chanduloy,

    1. Department of Oncology, Cancer Research Centre, Queen's University Belfast, Belfast City Hospital, Belfast BT9 7AB, N Ireland, UK
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    • These authors have contributed equally to this paper.

  • Michael A McIlhatton,

    1. Department of Oncology, Cancer Research Centre, Queen's University Belfast, Belfast City Hospital, Belfast BT9 7AB, N Ireland, UK
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  • Hans Nagar,

    1. Department of Oncology, Cancer Research Centre, Queen's University Belfast, Belfast City Hospital, Belfast BT9 7AB, N Ireland, UK
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  • Karen Yeates,

    1. Department of Oncology, Cancer Research Centre, Queen's University Belfast, Belfast City Hospital, Belfast BT9 7AB, N Ireland, UK
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  • Paul Donaghy,

    1. Department of Oncology, Cancer Research Centre, Queen's University Belfast, Belfast City Hospital, Belfast BT9 7AB, N Ireland, UK
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  • John Price,

    1. Department of Obstetrics and Gynaecology, Cancer Research Centre, Queen's University Belfast, Belfast City Hospital, Belfast BT9 7AB, N Ireland, UK
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  • Andrew K Godwin,

    1. Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, PA 19111, USA
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  • Patrick G Johnston,

    1. Department of Oncology, Cancer Research Centre, Queen's University Belfast, Belfast City Hospital, Belfast BT9 7AB, N Ireland, UK
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  • SE Hilary Russell

    Corresponding author
    1. Department of Oncology, Cancer Research Centre, Queen's University Belfast, Belfast City Hospital, Belfast BT9 7AB, N Ireland, UK
    • Department of Oncology, Cancer Research Centre, Queen's University Belfast, Belfast City Hospital, Belfast BT9 7AB, N Ireland, UK.
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Abstract

The septin family of genes has been implicated in a variety of cellular processes including cytokinesis, membrane transport and fusion, exocytosis, and apoptosis. One member of the septin family maps to chromosome 17q25.3, a region commonly deleted in sporadic ovarian and breast tumours, and has also been identified as a fusion partner of MLL in acute myeloid leukaemias. The present study demonstrates that the pattern of expression of multiple splice variants of this septin gene is altered in ovarian tumours and cell lines. In particular, expression of the zeta transcript is detectable in the majority of tumours and cell lines, but not in a range of non-malignant adult and fetal tissues. Zeta expression is accompanied by loss of the ubiquitous beta transcript. Somatic mutations of the gene were not detected in ovarian tumours, but it was demonstrated that beta expression in tumour cell lines can be reactivated by 5-azacytidine treatment, suggesting a role for methylation in the control of expression of this gene. Copyright © 2003 John Wiley & Sons, Ltd.

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