The results of this study were presented in part at the United States and Canadian Academy of Pathology Meeting, Washington, DC, USA, 22–28 March 2003.
Inflammatory malignant fibrous histiocytomas and dedifferentiated liposarcomas: histological review, genomic profile, and MDM2 and CDK4 status favour a single entity†
Article first published online: 21 JUN 2004
Copyright © 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
The Journal of Pathology
Volume 203, Issue 3, pages 822–830, July 2004
How to Cite
Coindre, J.-M., Hostein, I., Maire, G., Derré, J., Guillou, L., Leroux, A., Ghnassia, J.-P., Collin, F., Pedeutour, F. and Aurias, A. (2004), Inflammatory malignant fibrous histiocytomas and dedifferentiated liposarcomas: histological review, genomic profile, and MDM2 and CDK4 status favour a single entity. J. Pathol., 203: 822–830. doi: 10.1002/path.1579
- Issue published online: 21 JUN 2004
- Article first published online: 21 JUN 2004
- Manuscript Accepted: 9 MAR 2004
- Manuscript Revised: 6 MAR 2004
- Manuscript Received: 10 JAN 2004
- Ligue Nationale Contre le Cancer
- Committees of Charente-Maritime and Pyrénées Atlantiques
- Programme Hospitalier de Recherche Clinique du CHU de Nice. Grant Number: PHRC#01-04
- Association pour la Recherche sur le Cancer. Grant Number: ARC 5955
- inflammatory malignant fibrous histiocytoma;
- dedifferentiated liposarcoma;
Inflammatory malignant fibrous histiocytoma (inflammatory MFH) is a very rare tumour that occurs most often in the retroperitoneum. So far, it has been considered to be a special subtype of MFH. As it is now widely accepted that most retroperitoneal pleomorphic MFHs are dedifferentiated liposarcomas, the present study compared histological features, genomic profile (CGH analysis), and MDM2 and CDK4 status (immunohistochemistry, FISH, and quantitative PCR) in inflammatory MFHs from 12 patients and dedifferentiated liposarcomas that had an inflammatory MFH component from eight patients. Metaphase cytogenetic and FISH analyses were also performed on one inflammatory MFH. Histological review showed areas of well-differentiated liposarcoma in nine inflammatory MFHs. CGH analysis showed 12q13–15 amplification or gain in six of seven inflammatory MFHs and in seven of seven dedifferentiated liposarcomas. Immunohistochemistry showed positivity of tumour cells for MDM2 in every tumour in both groups and for CDK4 in ten and seven inflammatory MFHs and dedifferentiated liposarcomas, respectively. Metaphase cytogenetic and FISH analysis performed on one inflammatory MFH showed the presence of a supernumerary large marker chromosome and ring chromosome with high-level amplification of both MDM2 and CDK4 genes. FISH analysis on paraffin wax-embedded sections showed amplifications of MDM2 and CDK4 in seven of seven inflammatory MFHs and in seven of seven dedifferentiated liposarcomas. Quantitative PCR showed amplification of MDM2 in six and of CDK4 in seven of nine inflammatory MFHs. In conclusion, this study strongly suggests that most so-called inflammatory MFHs are dedifferentiated liposarcomas. Copyright © 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.