New monoclonal antibodies reacting with bile ducts: Further insights into the pathogenesis of bile ductular proliferation in biliary diseases
Version of Record online: 16 JUN 2005
Copyright © 1991 John Wiley & Sons, Ltd.
The Journal of Pathology
Volume 165, Issue 2, pages 153–161, October 1991
How to Cite
Ayres, R. C. S., Hübscher, S. G., Shaw, J., Garner, C., Joplin, R., Williams, A. and Neuberger, J. M. (1991), New monoclonal antibodies reacting with bile ducts: Further insights into the pathogenesis of bile ductular proliferation in biliary diseases. J. Pathol., 165: 153–161. doi: 10.1002/path.1711650211
- Issue online: 16 JUN 2005
- Version of Record online: 16 JUN 2005
- Manuscript Accepted: 29 APR 1991
- Manuscript Received: 20 DEC 1990
- Endowment Fund of the former United Birmingham Hospitals
- Monoclonal antibodies;
- biliary epithelial cells
We have produced a range of monoclonal antibodies which stain human intrahepatic bile ducts of different sizes. Amongst 26 monoclonal antibodies produced, five clones reacted specifically with bile ducts of different sizes, of which three have been maintained in culture and their viability following freezing and thawing confirmed. Staining patterns varied between normal adult liver tissue, normal fetal liver tissue and a variety of hepatobiliary diseases. The antibodies provide further evidence of the immunological heterogeneity of the human intrahepatic biliary tree and support the hypothesis that proliferating bile ductules are derived from periseptal hepatocytes. The preparation of the antiboides, their staining reactions in normal adult, normal fetal and a variety of liver diseases are described.