Immunolocalization of regenerating cells after submassive liver necrosis using PCNA staining
Article first published online: 15 JUN 2005
Copyright © 1992 John Wiley & Sons, Ltd.
The Journal of Pathology
Volume 166, Issue 4, pages 359–368, April 1992
How to Cite
Koukoulis, G., Rayner, A., Tan, K.-C., Williams, R. and Portmann, B. (1992), Immunolocalization of regenerating cells after submassive liver necrosis using PCNA staining. J. Pathol., 166: 359–368. doi: 10.1002/path.1711660407
- Issue published online: 15 JUN 2005
- Article first published online: 15 JUN 2005
- Manuscript Accepted: 1 NOV 1991
- Manuscript Received: 26 JUL 1991
- acute liver failure;
Little data exist on the proliferative state of liver cells and its relationship with various morphological findings in acute liver failure (ALF) in man. In this study we used the monoclonal antibody NCL-PCNA (clone PC-10) against the proliferating cell nuclear antigen (PCNA) to detect cycling cells in paraffin sections of 3 normal livers, 14 post-mortem needle-specimens of submassive hepatic necrosis (SHN) due to paracetamol overdosage (POD), and 10 hepatectomy specimens obtained at transplantation in patients with acute or subacute liver failure of presumed viral aetiology. In normal livers, only occasional sinusoid-lining cells were stained, whereas in SHN following POD or presumed viral hepatitis, hepatocytes of variable morphology showed significant immunoreactivity. Following POD, immuno-reactivity was higher in samples taken within 5–6 days than in those obtained at 9–11 days, a pattern reminiscent of the decrease in the rate of regeneration, previously documented after partial hepatectomy in humans. Immunolabelled hepatocytes were aggregated in multiacinar ‘nodules’ in cases with a map-like distribution of collapsed and non-collapsed parenchyma. Ductules demonstrated comparatively less staining, but extensive labelling was exceptionally found in areas of complete hepatocellular dropout. In these areas, small elongated cells with strongly PCNA-positive ovoid nuclei, forming periporal sprouting cords or incorporated into the lining of ductules, were most remarkable in that they closely resembled ‘oval cells’ described in animal studies.